Anticorps Polyclonal de lapin anti-ADH4

• Phare
• Validé par KD/KO

ADH4 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 16474-1-AP

Synonymes

ADH 2, ADH4, Alcohol dehydrogenase 4

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Galerie de données de validation

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  WB analysis of HepG2 using 16474-1-AP

  WB result of ADH4 antibody (16474-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ADH4 transfected HepG2 cells.

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  WB analysis using 16474-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16474-1-AP (ADH4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16474-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16474-1-AP (ADH4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 16474-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16474-1-AP (ADH4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 16474-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16474-1-AP (ADH4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 16474-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16474-1-AP (ADH4 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human heart using 16474-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16474-1-AP (ADH4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 16474-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16474-1-AP (ADH4 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HeLa, tissu cérébral de souris, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IHC: tissu rénal humain, tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 2 publications below

Informations sur le produit

16474-1-AP cible ADH4 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ADH4 Protéine recombinante Ag9568
• Nom complet: alcohol dehydrogenase 4 (class II), pi polypeptide
• Masse moléculaire calculée: 380 aa, 40 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC022319
• Symbole du gène: ADH4
• Identification du gène (NCBI): 127
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

This gene encodes the class II alcohol dehydrogenase 4 pi subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class II alcohol dehydrogenase is a homodimer composed of 2 pi subunits. ADH4 plays a critical role in alcoholism development and alcohol toxicology and is also associated with the development of esophageal cancer(PMID:33556337, 26039424, 24051444, 22147505).

Publications

Species	Application	Title
human	IHC	Front Immunol; Identification and validation of a tyrosine metabolism-related prognostic prediction model and characterization of the tumor microenvironment infiltration in hepatocellular carcinoma; Authors - Yangying Zhou
human	IHC	Front Oncol; Novel Prognostic Signatures of Hepatocellular Carcinoma Based on Metabolic Pathway Phenotypes.; Authors - Tingbo Ye