Anticorps Polyclonal de lapin anti-ADRA1B

ADRA1B Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(4)

Avis (2)

N° de cat : 22419-1-AP

Synonymes

ADRA1, Alpha 1B adrenergic receptor, Alpha 1B adrenoceptor, Alpha 1B adrenoreceptor, Alpha-1B adrenergic receptor

---

Galerie de données de validation

• 

  WB analysis using 22419-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22419-1-AP (ADRA1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of PC-3 using 22419-1-AP

  1x10^6 PC-3 cells were intracellularly stained with 0.8 ug ADRA1B Polyclonal antibody (22419-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cardiaque de souris, tissu cérébral de rat, tissu testiculaire de souris
• Résultats positifs en FC (Intra): cellules PC-3,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 2 publications below

Informations sur le produit

22419-1-AP cible ADRA1B dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ADRA1B Protéine recombinante Ag18138
• Nom complet: adrenergic, alpha-1B-, receptor
• Masse moléculaire calculée: 520 aa, 57 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC136569
• Symbole du gène: ADRA1B
• Identification du gène (NCBI): 147
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
rat	WB,IHC	Chin Med; Neurobehavioral alternations of the female offspring born to polycystic ovary syndrome model rats administered by Chinese herbal medicine.; Authors - Xian Zhang
rat	WB	Eur J Pharmacol; Alpha1B-adreneroceptor is involved in norepinephrine-induced pulmonary artery smooth muscle cell proliferation via p38 signaling; Authors - Xiaolin Xiao
human	WB	Mol Med Rep; Stigmasterol exerts antiglioma effects by regulating lipid metabolism; Authors - Ting Wei
human	IHC	Onco Targets Ther; Sympathetic innervation contributes to perineural invasion of salivary adenoid cystic carcinoma via the β2-adrenergic receptor.; Authors - Chao Ma

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Andrea (Verified Customer) (07-31-2023)

Good signal in Flow cytometry for endogenous expressing levels and confirmed in overexpressing cell line

• Applications: Flow Cytometry
• Primary Antibody Dilution: 0.4ug/ml

FH

Mohamad (Verified Customer) (07-03-2023)

different bands at different MW

• Applications: Western Blot
• Primary Antibody Dilution: 1/100
• Cell Tissue Type: Lung

{{ptg:RelatedPrimaryAntibodies}}