Anticorps Polyclonal de lapin anti-Adenylosuccinate lyase

Adenylosuccinate lyase Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 15264-1-AP

Synonymes

Adenylosuccinase, adenylosuccinate lyase, ADSL, AMPS, ASASE, ASL

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Galerie de données de validation

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  WB analysis using 15264-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15264-1-AP (Adenylosuccinate lyase antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15264-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15264-1-AP (Adenylosuccinate lyase antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15264-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15264-1-AP (Adenylosuccinate lyase antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 15264-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 15264-1-AP (Adenylosuccinate lyase antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 15264-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 15264-1-AP (Adenylosuccinate lyase antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 15264-1-AP

  Immunofluorescent analysis of Hela cells, using ADSL antibody 15264-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HeLa using 15264-1-AP

  1X10^6 HeLa cells were stained with 0.2ug Adenylosuccinate lyase antibody (15264-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules NIH/3T3, cellules RAW264.7
• Résultats positifs en IHC: tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

15264-1-AP cible Adenylosuccinate lyase dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Adenylosuccinate lyase Protéine recombinante Ag7332
• Nom complet: adenylosuccinate lyase
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC000253
• Symbole du gène: ADSL
• Identification du gène (NCBI): 158
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ADSL(adenylosuccinate lyase) is also named as AMPS, ASase, ASL and belongs to the lyase 1 family. It is an enzyme involved in 2 pathways of purine nucleotide metabolism and catalyzes cleavage of succinyl groups to yield fumarate(PMID:18524658). Defects in ADSL are the cause of adenylosuccinase deficiency (ADSL deficiency). In humans, mutations in ADSL lead to an inborn error of metabolism originally characterized by developmental delay, often with autistic features(PMID:20884265)..The ADSL enzymatic activity is reduced in lymphocytes and red blood cells of the patient with severe psychomotor retardation(PMID:9545543). It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Mol Cell; ERK2 Phosphorylates PFAS to Mediate Posttranslational Control of De Novo Purine Synthesis.; Authors - Eunus S Ali
human	WB, IHC	Front Cell Dev Biol; Thyroid Hormone-Regulated Expression of Period2 Promotes Liver Urate Production.; Authors - Xiaoting Chen
human	WB	Cell Death Differ; Oxoglutarate dehydrogenase-like inhibits the progression of hepatocellular carcinoma by inducing DNA damage through non-canonical function; Authors - Xiang Jiang