Anticorps Polyclonal de lapin anti-AFF4

• Phare
• Validé par KD/KO

AFF4 Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 14662-1-AP

Synonymes

AF4/FMR2 family member 4, AF4/FMR2 family, member 4, AF5Q31, AFF4, MCEF, Protein AF 5q31

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Galerie de données de validation

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  WB analysis using 14662-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14662-1-AP (AFF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14662-1-AP

  IP result of anti-AFF4(IP:14662-1-AP, 4ug; Detection:14662-1-AP 1:20000) with HeLa cells lysate 1880 ug.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IHC: See 1 publications below
• IP: See 2 publications below

Informations sur le produit

14662-1-AP cible AFF4 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AFF4 Protéine recombinante Ag6336
• Nom complet: AF4/FMR2 family, member 4
• Masse moléculaire calculée: 127 kDa
• Poids moléculaire observé: 140-150 kDa
• Numéro d’acquisition GenBank: BC063007
• Symbole du gène: AFF4
• Identification du gène (NCBI): 27125
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Transcriptional controled by RNA polymerase II (Pol II) is a multi-step process requiring the concerted action of multiple factors and contacts with the DNA template for the proper synthesis of nascent RNA [PMID:12676794]. ELL1 was demonstrated to be a Pol II elongation factor capable of enhancing the catalytic rate of transcription elongation by reducing transient pausing by the enzyme [PMID:8596958]. AFF4 was initially identified as a fusion partner of the MLL (mixed-lineage leukemia) gene participated in infant acute lymphoblastic leukemia, and a component of the ELL/p-TEFb elongation complex. AFF4 interacts with positive transcription elongation factor-b while also repressing Tat transactivation of HIV-1 [PMID:17389929].

Publications

Species	Application	Title
human	WB	Cell; Targeting Processive Transcription Elongation via SEC Disruption for MYC-Induced Cancer Therapy.; Authors - Kaiwei Liang
mouse	WB	Nat Commun; Transcriptional repression by a secondary DNA binding surface of DNA topoisomerase I safeguards against hypertranscription; Authors - Mei Sheng Lau
human,mouse	WB	Mol Cell; RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits; Authors - Yuanjun Li
human	WB	Genes Dev; Acute perturbation strategies in interrogating RNA polymerase II elongation factor function in gene expression.; Authors - Bin Zheng
human	WB	Nucleic Acids Res; Multiple P-TEFbs cooperatively regulate the release of promoter-proximally paused RNA polymerase II.; Authors - Xiaodong Lu ; KD Validated
human	WB	Cell Discov; Structural and functional insight into the effect of AFF4 dimerization on activation of HIV-1 proviral transcription; Authors - Dan Tang