Anticorps Polyclonal de lapin anti-AFP

AFP Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

95

N° de cat : 14550-1-AP

Synonymes

AFP, Alpha 1 fetoprotein, Alpha fetoglobulin, alpha fetoprotein, FETA, HPAFP

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Galerie de données de validation

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  WB analysis using 14550-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14550-1-AP (AFP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 14550-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14550-1-AP (AFP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 14550-1-AP

  IP Result of anti-AFP (IP:14550-1-AP, 5ug; Detection:14550-1-AP 1:1000) with HepG2 cells lysate 6000ug.

• 

  IHC staining of human liver cancer using 14550-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14550-1-AP (AFP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 14550-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14550-1-AP (AFP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 14550-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14550-1-AP (AFP antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver cancer using 14550-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14550-1-AP (AFP antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver cancer using 14550-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14550-1-AP (AFP antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver cancer using 14550-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14550-1-AP (AFP antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary tumor using 14550-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14550-1-AP (AFP Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human ovary tumor using 14550-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14550-1-AP (AFP Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of MCF-7 using 14550-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 14550-1-AP (AFP antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules BxPC-3, cellules HuH-7, cellules L02, tissu placentaire humain
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 28 publications below
• IHC: See 39 publications below
• IF: See 31 publications below
• FC: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

14550-1-AP cible AFP dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AFP Protéine recombinante Ag6089
• Nom complet: alpha-fetoprotein
• Masse moléculaire calculée: 69 kDa
• Poids moléculaire observé: 68-72 kDa
• Numéro d’acquisition GenBank: BC027881
• Symbole du gène: AFP
• Identification du gène (NCBI): 174
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AFP, also known as Alpha-fetoprotein, is a major plasma protein in the fetus and its concentration is very low in the adult (PubMed:24120489). AFP is considered a marker of hepatocyte precursors. AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma. High AFP concentrations have been correlated with tumor cell growth, indicating that AFP can serve as a tumor marker (PubMed: 18669658).

Publications

Species	Application	Title
mouse	IHC	Cell; Glycogen accumulation and phase separation drives liver tumor initiation.; Authors - Qingxu Liu
human	IHC	Cancer Cell; Genomic and Transcriptomic Profiling of Combined Hepatocellular and Intrahepatic Cholangiocarcinoma Reveals Distinct Molecular Subtypes.; Authors - Ruidong Xue
mouse	IHC	J Clin Invest; Hepatocellular carcinoma originates from hepatocytes and not from the progenitor/biliary compartment.; Authors - Xueru Mu
human	IHC	J Clin Invest; HMGB1 links chronic liver injury to progenitor responses and hepatocarcinogenesis.; Authors - Celine Hernandez
human,mouse	WB,IHC,IF	Nat Commun; Growth differentiation factor 1-induced tumour plasticity provides a therapeutic window for immunotherapy in hepatocellular carcinoma.; Authors - Wei Cheng
human	IHC	Nat Commun; Whole-exome sequencing of alpha-fetoprotein producing gastric carcinoma reveals genomic profile and therapeutic targets.; Authors - Jun Lu

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