Anticorps Polyclonal de lapin anti-AGGF1

• Phare
• Validé par KD/KO

AGGF1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

20

N° de cat : 11889-1-AP

Synonymes

AGGF1, Angiogenic factor VG5Q, GPATC7, GPATCH7, HSU84971, HUS84971, hVG5Q, VG5Q

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Galerie de données de validation

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  WB analysis of HeLa using 11889-1-AP

  WB result of AGGF1 antibody (11889-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AGGF1 transfected HeLa cells.

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  WB analysis using 11889-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11889-1-AP (AGGF1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HUVEC, cellules HeLa, cellules L02

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 7 publications below
• WB: See 16 publications below
• IHC: See 7 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Informations sur le produit

11889-1-AP cible AGGF1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris, singe Rhésus, Macaque
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AGGF1 Protéine recombinante Ag2497
• Nom complet: angiogenic factor with G patch and FHA domains 1
• Masse moléculaire calculée: 714 aa, 81 kDa
• Poids moléculaire observé: 84-100 kDa
• Numéro d’acquisition GenBank: BC029382
• Symbole du gène: AGGF1
• Identification du gène (NCBI): 55109
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The angiogenic factor gene, AGGF1 (or VG5Q), is identified as a candidate susceptibility gene for Klippel-Trenaunay syndrome (KTS) which is a severe congenital disorder characterized by capillary malformations, venous malformations or varicose veins, and hypertrophy of the affected tissues. AGGF1 protein can bind to endothelial cells and promote cell proliferation. AGGF1 shows strong expression in blood vessels and is secreted as vessel formation is initiated. Regulation of AGGF1 by GATA1 may play roles in endothelial cell biology and angiogenesis. Catalog#11889-1-AP was validated by KO and KD methods in scientists' studies (PMID: 28153879; 27513923), and the observed MW is 84 kDa.

Publications

Species	Application	Title
human	WB,IHC	Nat Commun; A non-canonical pathway regulates ER stress signaling and blocks ER stress-induced apoptosis and heart failure.; Authors - Yufeng Yao
mouse	WB	Mol Ther; The 3' Untranslated Region Protects the Heart from Angiotensin II-Induced Cardiac Dysfunction via AGGF1 Expression.; Authors - Lexi Ding
mouse	WB	PLoS Biol; Angiogenic Factor AGGF1 Activates Autophagy with an Essential Role in Therapeutic Angiogenesis for Heart Disease.; Authors - Qiulun Lu ; KO Validated
rat	WB,IF	Arterioscler. Thromb. Vasc. Biol.; Angiogenic Factor With G Patch and FHA Domains 1 Is a Novel Regulator of Vascular Injury.; Authors - Bisheng Zhou ; KD Validated
human	WB	Mol Ther Nucleic Acids; circ-SHKBP1 Regulates the Angiogenesis of U87 Glioma-Exposed Endothelial Cells through miR-544a/FOXP1 and miR-379/FOXP2 Pathways.; Authors - Qianru He
		FASEB J; Angiotensin II increases angiogenesis by NF-κB-mediated transcriptional activation of angiogenic factor AGGF1.; Authors - Wenxia Si

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