Anticorps Recombinant de lapin anti-AGPAT2

AGPAT2 Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240153C2

N° de cat : 83349-3-RR

Synonymes

240153C2, 1-AGPAT 2, 1-AGP acyltransferase 2, 1-acyl-sn-glycerol-3-phosphate acyltransferase beta, 1-acylglycerol-3-phosphate O-acyltransferase 2

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Galerie de données de validation

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  WB analysis using 83349-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83349-3-RR (AGPAT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 83349-3-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83349-3-RR (AGPAT2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of A549 using 83349-3-RR

  1x10^6 A549 cells were intracellularly stained with 0.25 ug AGPAT2 Recombinant antibody (83349-3-RR, Clone:240153C2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83349-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83349-3-RR against Human AGPAT2 were performed. The affinity constant is 2.72 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, cellules THP-1
• Résultats positifs en IHC: human ovary cancer tissue, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83349-3-RR cible AGPAT2 dans les applications de WB, IHC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: AGPAT2 Protéine recombinante Ag34761
• Nom complet: 1-acylglycerol-3-phosphate O-acyltransferase 2 (lysophosphatidic acid acyltransferase, beta)
• Masse moléculaire calculée: 31 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC019292
• Symbole du gène: AGPAT2
• Identification du gène (NCBI): 10555
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

1-acyl-sn-glycerol-3-phosphate acyltransferase beta (AGPAT2) belongs to a family of enzymes catalyzing the sn-2 acylation of the glycerol-3-phosphate backbone. AGPAT2 is highly expressed in adipose tissues, liver, and skeletal muscle. AGPAT2 is the only AGPAT isoform whose loss-of-function mutations cause a severe form of human congenital generalized lipodystrophy (PMID: 34824276). Human and mouse AGPAT2 have a calculated molecular mass of 31 kDa, and an alternatively spliced form of AGPAT2 mRNA, encoding a protein of 246 rather than 278 amino acids, is also found in human (PMID: 19336658). Western detected AGPAT2 at an apparent molecular mass of 27 kDa.

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