Anticorps Polyclonal de lapin anti-AGR2

• Phare
• Validé par KD/KO

AGR2 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

14

N° de cat : 12275-1-AP

Synonymes

AG 2, AG2, AGR2, GOB 4, HAG 2, HPC8, PDIA17, XAG 2

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Galerie de données de validation

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  WB analysis using 12275-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12275-1-AP (AGR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12275-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12275-1-AP (AGR2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat colon using 12275-1-AP

  rat colon tissue were subjected to SDS PAGE followed by western blot with 12275-1-AP (AGR2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 12275-1-AP

  IP result of anti-AGR2(IP:12275-1-AP, 4ug; Detection:12275-1-AP 1:1000) with A549 cells lysate 1200 ug.

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  IHC staining of human breast cancer using 12275-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12275-1-AP (AGR2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 12275-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12275-1-AP (AGR2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 12275-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 12275-1-AP (AGR2 Antibody) at dilution of 1:50.

• 

  IHC staining of human colon using 12275-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 12275-1-AP (AGR2 Antibody) at dilution of 1:50.

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  FC experiment of MCF-7 using 12275-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug AGR2 antibody (12275-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: cellules A549, tissu de côlon de rat, tissu d'estomac de rat, tissu d'estomac de souris
• Résultats positifs en IP: cellules A549,
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 8 publications below
• IF: See 2 publications below

Informations sur le produit

12275-1-AP cible AGR2 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AGR2 Protéine recombinante Ag2919
• Nom complet: anterior gradient homolog 2 (Xenopus laevis)
• Masse moléculaire calculée: 175 aa, 20 kDa
• Poids moléculaire observé: 17-20 kDa
• Numéro d’acquisition GenBank: BC015503
• Symbole du gène: AGR2
• Identification du gène (NCBI): 10551
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AGR2, also named AG2 or HPC8, encodes anterior gradient protein 2 homolog which belongs to the AGR family. It is a secreted protein localized in endoplasmic reticulum. AGR2 plays roles in MUC2 post-transcriptional synthesis,secretion and production of mucus by intestinal cells. AGR2 was significantly elevated in the pancreatic juice from patients with pre-malignant conditions as well as pancreatic cancer compared to control pancreatic juice samples. AGR2 levels in pancreatic juice could potentially be used in assessment of high-risk patients undergoing endoscopic procedures.

Publications

Species	Application	Title
human	IF	Nat Cell Biol; Opposing Wnt signals regulate cervical squamocolumnar homeostasis and emergence of metaplasia.; Authors - Cindrilla Chumduri
human,mouse	IHC	Nat Protoc; Patient-derived and mouse endo-ectocervical organoid generation, genetic manipulation and applications to model infection.; Authors - Rajendra Kumar Gurumurthy
human	WB,IHC	Proc Natl Acad Sci U S A; A discrete population of squamocolumnar junction cells implicated in the pathogenesis of cervical cancer.; Authors - Herfs Michael M
human	IHC	J Pathol; A novel blueprint for 'top down' differentiation defines the cervical squamocolumnar junction during development, reproductive life, and neoplasia.; Authors - Michael Herfs
human	IHC	Int J Cancer; Unique recurrence patterns of cervical intraepithelial neoplasia following excision of the squamo-columnar junction.; Authors - Michael Herfs
human	WB	J Proteome Res; Identification of serum biomarkers for colorectal cancer metastasis using a differential secretome approach.; Authors - Xue Hua H

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