Anticorps Polyclonal de lapin anti-AGR2

• Phare
• Validé par KD/KO

AGR2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications(22)

N° de cat : 12275-1-AP

Synonymes

AG 2, AG2, AG-2, AGR 2, GOB 4

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Galerie de données de validation

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  WB analysis using 12275-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12275-1-AP (AGR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat colon using 12275-1-AP

  rat colon tissue were subjected to SDS PAGE followed by western blot with 12275-1-AP (AGR2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12275-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12275-1-AP (AGR2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 12275-1-AP

  IP result of anti-AGR2 (IP:12275-1-AP, 4ug; Detection:12275-1-AP 1:1000) with A549 cells lysate 1200 ug.

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  IHC staining of human breast cancer using 12275-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12275-1-AP (AGR2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 12275-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12275-1-AP (AGR2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 12275-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 12275-1-AP (AGR2 Antibody) at dilution of 1:50.

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  IHC staining of human colon using 12275-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 12275-1-AP (AGR2 Antibody) at dilution of 1:50.

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  IF Staining of A549 using 12275-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using AGR2 antibody (12275-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of A549 using 12275-1-AP

  1x10^6 A549 cells were intracellularly stained with 0.4 ug AGR2 Polyclonal antibody (12275-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A549, tissu de côlon de rat, tissu d'estomac de rat, tissu d'estomac de souris
• Résultats positifs en IP: cellules A549,
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549,
• Résultats positifs en FC (Intra): cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IHC: See 9 publications below
• IF: See 7 publications below
• IP: See 1 publications below

Informations sur le produit

12275-1-AP cible AGR2 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AGR2 Protéine recombinante Ag2919
• Nom complet: anterior gradient homolog 2 (Xenopus laevis)
• Masse moléculaire calculée: 175 aa, 20 kDa
• Poids moléculaire observé: 17-20 kDa
• Numéro d’acquisition GenBank: BC015503
• Symbole du gène: AGR2
• Identification du gène (NCBI): 10551
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AGR2, also named AG2 or HPC8, encodes anterior gradient protein 2 homolog which belongs to the AGR family. It is a secreted protein localized in endoplasmic reticulum. AGR2 plays roles in MUC2 post-transcriptional synthesis,secretion and production of mucus by intestinal cells. AGR2 was significantly elevated in the pancreatic juice from patients with pre-malignant conditions as well as pancreatic cancer compared to control pancreatic juice samples. AGR2 levels in pancreatic juice could potentially be used in assessment of high-risk patients undergoing endoscopic procedures.

Publications

Species	Application	Title
human	IF	Nat Cell Biol; Opposing Wnt signals regulate cervical squamocolumnar homeostasis and emergence of metaplasia.; Authors - Cindrilla Chumduri
mouse,human	IHC	Nat Protoc; Patient-derived and mouse endo-ectocervical organoid generation, genetic manipulation and applications to model infection.; Authors - Rajendra Kumar Gurumurthy
human	IHC	Cell Rep Med; Distinctive multicellular immunosuppressive hubs confer different intervention strategies for left- and right-sided colon cancers; Authors - Bingxin Liu
human	WB,IHC	Proc Natl Acad Sci U S A; A discrete population of squamocolumnar junction cells implicated in the pathogenesis of cervical cancer.; Authors - Herfs Michael M
human	IHC	J Pathol; A novel blueprint for 'top down' differentiation defines the cervical squamocolumnar junction during development, reproductive life, and neoplasia.; Authors - Michael Herfs
human	IHC	Int J Cancer; Unique recurrence patterns of cervical intraepithelial neoplasia following excision of the squamo-columnar junction.; Authors - Michael Herfs

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