Anticorps Polyclonal de lapin anti-CCP1

• Phare
• Validé par KD/KO

CCP1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

15

N° de cat : 14067-1-AP

Synonymes

AGTPBP1, ATP/GTP binding protein 1, CCP1, Cytosolic carboxypeptidase 1, KIAA1035, NNA1

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Galerie de données de validation

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  WB analysis of mouse brain using 14067-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14067-1-AP (CCP1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 14067-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14067-1-AP (CCP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 14067-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14067-1-AP (CCP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 14067-1-AP

  IP Result of anti-CCP1 (IP:14067-1-AP, 3ug; Detection:14067-1-AP 1:1000) with mouse testis tissue lysate 10000ug.

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  IHC staining of human testis using 14067-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14067-1-AP (CCP1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of mouse testis using 14067-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14067-1-AP (CCP1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 14067-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14067-1-AP (CCP1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 14067-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14067-1-AP (CCP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 14067-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14067-1-AP (CCP1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 14067-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14067-1-AP (CCP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 14067-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14067-1-AP (CCP1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A549 using 14067-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 14067-1-AP (CCP1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules A549, tissu testiculaire de souris
• Résultats positifs en IP: tissu testiculaire de souris
• Résultats positifs en IHC: tissu testiculaire humain, tissu cardiaque humain, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IHC: See 3 publications below
• IF: See 6 publications below
• IP: See 1 publications below

Informations sur le produit

14067-1-AP cible CCP1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CCP1 Protéine recombinante Ag5122
• Nom complet: ATP/GTP binding protein 1
• Masse moléculaire calculée: 138 kDa
• Poids moléculaire observé: 138 kDa
• Numéro d’acquisition GenBank: BC060815
• Symbole du gène: AGTPBP1
• Identification du gène (NCBI): 23287
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AGTPBP1, also known as CCP1 (cytosolic carboxy-peptidase 1), or Nna1, belongs to the peptidase M14 family. AGTPBP1 is a zinc carboxypeptidase that contains nuclear localization signals and an ATP/GTP-binding motiff. Thus, it may contribute to nuclear signaling events in differentiating and regenerating neurons. It serves important functions in the nervous system, and AGTPBP1 mutations may cause Purkinje cell degeneration (pcd) phenotypes.

Publications

Species	Application	Title
mouse	IF	Cell; Cell-Intrinsic Control of Interneuron Migration Drives Cortical Morphogenesis.; Authors - Carla G Silva
mouse	WB	Nat Commun; Klf4 glutamylation is required for cell reprogramming and early embryonic development in mice.; Authors - Buqing Ye ; KO Validated
human,mouse	WB,IF	EMBO J; Loss of tubulin deglutamylase CCP1 causes infantile-onset neurodegeneration.; Authors - Vandana Shashi
mouse	WB	Nat Commun; AMPK activation promotes lipid droplet dispersion on detyrosinated microtubules to increase mitochondrial fatty acid oxidation.; Authors - Albert Herms
rat	WB	Proc Natl Acad Sci U S A; Pathogenic role of delta 2 tubulin in bortezomib-induced peripheral neuropathy.; Authors - Maria Elena Pero ; KO Validated
human,mouse	WB,IP	J Cell Biol; CCP1 promotes mitochondrial fusion and motility to prevent Purkinje cell neuron loss in pcd mice.; Authors - Stephen Gilmore-Hall