Anticorps Polyclonal de lapin anti-AHCY

• Phare
• Validé par KD/KO

AHCY Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

24

Avis (1)

N° de cat : 10757-2-AP

Synonymes

Adenosylhomocysteinase, AdoHcyase, AHCY, S adenosylhomocysteine hydrolase, SAHH

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Galerie de données de validation

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  WB analysis using 10757-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10757-2-AP (AHCY antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10757-2-AP

  WB result of AHCY antibody (10757-2-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AHCY transfected HeLa cells.

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  WB analysis of mouse liver using 10757-2-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10757-2-AP (AHCY antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10757-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10757-2-AP (AHCY Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 10757-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10757-2-AP (AHCY antibody) at dilution of 1:100 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules HeLa, cellules HepG2, cellules Jurkat, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 20 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

10757-2-AP cible AHCY dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AHCY Protéine recombinante Ag1208
• Nom complet: S-adenosylhomocysteine hydrolase
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 45-48 kDa
• Numéro d’acquisition GenBank: BC011606
• Symbole du gène: AHCY
• Identification du gène (NCBI): 191
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AHCY, also named as SAHH, belongs to the adenosylhomocysteinase family. AHCY is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore AHCY may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine. AHCY catalyzes the reversible hydrolysis of S-adenosylhomocysteine to adenosine and L-homocysteine.(PMID:21220475).

Publications

Species	Application	Title
human	WB	Cell Stem Cell; Disrupting Mitochondrial Copper Distribution Inhibits Leukemic Stem Cell Self-Renewal.; Authors - Rashim Pal Singh
human	WB, IP	Nat Commun; H19 lncRNA alters DNA methylation genome wide by regulating S-adenosylhomocysteine hydrolase.; Authors - Jichun Zhou ; KD Validated
human	WB	Autophagy; AHCYL1 senses SAH to inhibit autophagy through interaction with PIK3C3 in an MTORC1-independent manner.; Authors - Wei Huang ; KD Validated
human	WB	Oncogene; PMID: 27775072; Authors - T Zhong
human	WB,IHC	Clin Cancer Res; Digital transcript profile analysis with aRNA-LongSAGE validates FERMT1 as a potential novel prognostic marker for colon cancer.; Authors - Fan Junwei J
mouse	WB	Arterioscler Thromb Vasc Biol; Increased plasma S-adenosylhomocysteine-accelerated atherosclerosis is associated with epigenetic regulation of endoplasmic reticulum stress in apoE-/- mice.; Authors - Yunjun Xiao ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kamal (Verified Customer) (01-26-2024)

Antibody worked well for western blot analysis when diluted with 1X TBST at the provided dilution.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Mouse liver tissue