Anticorps Polyclonal de lapin anti-AHI1

• Phare
• Validé par KD/KO

AHI1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 22045-1-AP

Synonymes

AHI 1, AHI1, JBTS3, Jouberin, ORF1

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Galerie de données de validation

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  WB analysis using 22045-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22045-1-AP (AHI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules Jurkat

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 6 publications below

Informations sur le produit

22045-1-AP cible AHI1 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AHI1 Protéine recombinante Ag16885
• Nom complet: Abelson helper integration site 1
• Masse moléculaire calculée: 1196 aa, 137 kDa
• Poids moléculaire observé: 137-150 kDa
• Numéro d’acquisition GenBank: BC094800
• Symbole du gène: AHI1
• Identification du gène (NCBI): 54806
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AHI1, encoding the Jouberin protein, is an alternatively spliced signaling molecule that contains seven Trp-Asp (WD) repeats, an SH3 domain, and numerous SH3-binding sites. AHI1 is expressed strongly in embryonic hindbrain and forebrain. AHI1 is involved in vesicle trafficking and is required for ciliogenesis, formation of primary non-motile cilium, and recruitment of RAB8A to the basal body of primary cilium. AHI1, as a positive modulator of classical Wnt signaling, may play a key role in ciliary signaling during cerebellar embryonic development (PMID: 15467982, PMID: 21623382).

Publications

Species	Application	Title
human	IF	Cell Rep; A CEP104-CSPP1 Complex Is Required for Formation of Primary Cilia Competent in Hedgehog Signaling.; Authors - Kari-Anne M Frikstad
human	IF	Front Cell Dev Biol; Superresolution Microscopy Reveals Distinct Phosphoinositide Subdomains Within the Cilia Transition Zone.; Authors - Sarah E Conduit
mouse	IF,WB	Mol Biol Cell; Cby1 promotes Ahi1 recruitment to a ring-shaped domain at the centriole-cilium interface and facilitates proper cilium formation and function.; Authors - Yin Loon Lee ; KD Validated
human	IF	Sci Rep; Targeted exon skipping rescues ciliary protein composition defects in Joubert syndrome patient fibroblasts.; Authors - Elisa Molinari
human	WB	FEBS Open Bio; Proximity-dependent biotinylation reveals an interaction between ubiquitin-specific peptidase 46 and centrosome-related proteins; Authors - Kazuma Yoshioka
mouse	IF	bioRxiv; Ciliopathy-associated protein, CEP290, is required for ciliary necklace and outer segment membrane formation in retinal photoreceptors; Authors - Abigail R Moye

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