Anticorps Polyclonal de lapin anti-AIP/ARA9

AIP/ARA9 Polyclonal Antibody for FC, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, FC

Conjugaison

Non conjugué

Publications

1

N° de cat : 18176-1-AP

Synonymes

AIP, ARA9, FKBP16, FKBP37, HBV X associated protein 2, Immunophilin homolog ARA9, XAP 2, XAP2

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Galerie de données de validation

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  WB analysis of HepG2 using 18176-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 18176-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 18176-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 18176-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 18176-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18176-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 18176-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 18176-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18176-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 18176-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 18176-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 18176-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 18176-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 18176-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 18176-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 18176-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 18176-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of HeLa using 18176-1-AP

  1X10^6 HeLa cells were stained with 0.2ug AIP/ARA9 antibody (18176-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HeLa, cellules NIH/3T3
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IP: See 1 publications below

Informations sur le produit

18176-1-AP cible AIP/ARA9 dans les applications de WB, IP, IHC, FC et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AIP/ARA9 Protéine recombinante Ag12869
• Nom complet: aryl hydrocarbon receptor interacting protein
• Masse moléculaire calculée: 330 aa, 38 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: BC104797
• Symbole du gène: AIP
• Identification du gène (NCBI): 9049
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	IP	J Clin Invest; Anakinra restores cellular proteostasis by coupling mitochondrial redox balance to autophagy; Authors - Frank L. van de Veerdonk