Anticorps Monoclonal anti-AK2

• Phare
• Validé par KD/KO

AK2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

3H2C10

N° de cat : 66127-1-Ig

Synonymes

3H2C10, adenylate kinase 2, Adenylate kinase 2, mitochondrial, N-terminally processed, Adenylate monophosphate kinase, ADK2

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Galerie de données de validation

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  WB analysis using 66127-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66127-1-Ig (AK2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis of HeLa using 66127-1-Ig

  WB result of AK2 antibody (66127-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AK2 transfected HeLa cells.

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  WB analysis of HEK-293 using 66127-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66127-1-Ig (AK2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 66127-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver slide using 66127-1-Ig (AK2 Antibody) at dilution of 1:50.

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  IHC staining of human kidney using 66127-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 66127-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66127-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66127-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 66127-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 66127-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat liver using 66127-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 66127-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66127-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66127-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 66127-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 66127-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat heart using 66127-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat heart using 66127-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 66127-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart slide using 66127-1-Ig (AK2 Antibody) at dilution of 1:50.

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  IHC staining of human heart using 66127-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart slide using 66127-1-Ig (AK2 Antibody) at dilution of 1:50.

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  IF Staining of HeLa using 66127-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AK2 antibody (66127-1-Ig, Clone: 3H2C10 ) at dilution of 1:5024 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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  IF Staining of HepG2 using 66127-1-Ig

  Immunofluorescent analysis of HepG2 cells using 66127-1-Ig (AK2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG.

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  IF Staining of HeLa using 66127-1-Ig

  Immunofluorescent analysis of HeLa cells using 66127-1-Ig (AK2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

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  FC experiment of HepG2 using 66127-1-Ig

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug AK2 Monoclonal antibody (66127-1-Ig, Clone:3H2C10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules 4T1, cellules HEK-293, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules NIH/3T3, cellules PC-12
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu cardiaque de rat, tissu cardiaque de souris, tissu cardiaque humain, tissu de cancer du foie humain, tissu hépatique humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules HeLa
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66127-1-Ig cible AK2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: AK2 Protéine recombinante Ag17911
• Nom complet: adenylate kinase 2
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 29 kDa
• Numéro d’acquisition GenBank: BC009405
• Symbole du gène: AK2
• Identification du gène (NCBI): 204
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AK2(Adenylate kinase 2, mitochondrial ) is also named as ADK2 and belongs to the adenylate kinase family. It plays a unique role in energy metabolism and energy transfer by regulating the ATP/ADP rate between the cytoplasma matrix and the mitochondria and it is increased expression during adipocyte and B cell differentiation(PMID:23020757). AK2 catalyzes the reversible transfer of a phosphoryl group from ATP to AMP(PMID:19043417). Defects in AK2 are the cause of reticular dysgenesis (RDYS)(PMID:19043416). It has 6 isoforms produced by alternative splicing.

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