Anticorps Polyclonal de lapin anti-AKR1B1

• Phare
• Validé par KD/KO

AKR1B1 Polyclonal Antibody for IF, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

8

Avis (1)

N° de cat : 15439-1-AP

Synonymes

ADR, AKR1B1, Aldehyde reductase, Aldose reductase, ALDR1, ALR2, AR

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Galerie de données de validation

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  WB analysis using 15439-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15439-1-AP (AKR1B1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 15439-1-AP

  WB result of AKR1B1 antibody (15439-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKR1B1 transfected A431 cells.

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  WB analysis of L02 using 15439-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 15439-1-AP (AKR1B1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 15439-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15439-1-AP (AKR1B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 15439-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 15439-1-AP (AKR1B1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 15439-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 15439-1-AP (AKR1B1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules BxPC-3, cellules HepG2, cellules L02, tissu hépatique humain, tissu placentaire humain
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IHC: See 1 publications below

Informations sur le produit

15439-1-AP cible AKR1B1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AKR1B1 Protéine recombinante Ag7142
• Nom complet: aldo-keto reductase family 1, member B1 (aldose reductase)
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC000260
• Symbole du gène: AKR1B1
• Identification du gène (NCBI): 231
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
rat	WB	Oxid Med Cell Longev; WJ-39, an Aldose Reductase Inhibitor, Ameliorates Renal Lesions in Diabetic Nephropathy by Activating Nrf2 Signaling.; Authors - Xiaoyu Zhou
human	WB	Biochem Pharmacol; Regulation on SIRT1-PGC-1α/Nrf2 pathway together with selective inhibition of aldose reductase makes compound hr5F a potential agent for the treatment of diabetic complications.; Authors - Zhihua Wang
rat	WB	Biomed Pharmacother; Systematic investigation of the pharmacological mechanism for renal protection by the leaves of Eucommia ulmoides Oliver using UPLC-Q-TOF/MS combined with network pharmacology analysis.; Authors - Qi Huang
rat	WB	Toxicol Appl Pharmacol; Rosmarinic acid exerts a neuroprotective effect on spinal cord injury by suppressing oxidative stress and inflammation via modulating the Nrf2/HO-1 and TLR4/NF-κB pathways.; Authors - Zhanjun Ma
human	WB	BMC Nephrol; Bioinformatics analysis of proteomics profiles in senescent human primary proximal tubule epithelial cells.; Authors - Yang Lu
human	WB	Curr Issues Mol Biol; AKR1B1 Represses Glioma Cell Proliferation through p38 MAPK-Mediated Bcl-2/BAX/Caspase-3 Apoptotic Signaling Pathways; Authors - Yu-Kai Huang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Parijat (Verified Customer) (01-12-2024)

Good antibody for studying hypertonic stress response.

• Applications: Flow Cytometry
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: IMCD3 cells