Anticorps Monoclonal anti-ALCAM

ALCAM Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

CoraLite® Plus 555 Fluorescent Dye

CloneNo.

3A6

N° de cat : CL555-65223

Synonymes

ALCAM, CD166, CD166 antigen, FLJ38514, MEMD

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Galerie de données de validation

• 

  FC experiment of human PBMCs using CL555-65223

  1X10^6 PHA-treated (day 3) human PBMCs were surface stained with 5 ul CoraLite® Plus 555 Anti-Human ALCAM (CL555-65223, Clone:3A6) (red), or unstained (blue). The black dashed line indicates unactivated PBMCs surface stained with 5 ul CoraLite® Plus 555 Anti-Human ALCAM (CL555-65223, Clone:3A6). Cells were not fixed. Monocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: PHA-treated human PBMCs

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL555-65223 cible ALCAM dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cultured human thymic epithelial cells
• Nom complet: activated leukocyte cell adhesion molecule
• Masse moléculaire calculée: 105 kDa
• Numéro d’acquisition GenBank: BC057809
• Symbole du gène: ALCAM
• Identification du gène (NCBI): 214
• Conjugaison: CoraLite® Plus 555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 554 nm / 570 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues and cell types, including activated T cells and monocytes, epithelial cells, fibroblasts, neuronal cells, hepatocytes, and bone marrow mesenchymal stem cells (PMID: 7760007; 25221999). Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759).