Anticorps Polyclonal de lapin anti-ALDH1A1-specific

• Phare
• Validé par KD/KO

ALDH1A1-specific Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 22109-1-AP

Synonymes

ALDC, ALDH E1, ALDH1, ALDH11, ALDH1A1, ALHDII, PUMB1, RALDH 1, RALDH1, Retinal dehydrogenase 1

---

Galerie de données de validation

• 

  WB analysis of K-562 using 22109-1-AP

  WB result of ALDH1A1 antibody (22109-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALDH1A1 transfected K-562 cells.

• 

  WB analysis of human liver using 22109-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 22109-1-AP (ALDH1A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human adrenal gland using 22109-1-AP

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 22109-1-AP (ALDH1A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 22109-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 22109-1-AP (ALDH1A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 22109-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 22109-1-AP (ALDH1A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 22109-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 22109-1-AP (ALDH1A1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 22109-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 22109-1-AP (ALDH1A1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human hepatocirrhosis using 22109-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 22109-1-AP (ALDH1A1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 22109-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 22109-1-AP (ALDH1A1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 22109-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22109-1-AP (ALDH1A1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 22109-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22109-1-AP (ALDH1A1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of A549 using 22109-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 22109-1-AP (ALDH1A1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 22109-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human ALDH1A1-specific (22109-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

---

Applications testées

• Résultats positifs en WB: tissu hépatique humain, cellules K-562, tissu de glande surrénale humain, tissu hépatique de souris
• Résultats positifs en IHC: tissu hépatique humain, tissu de cirrhose hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A549,
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

22109-1-AP cible ALDH1A1-specific dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ALDH1A1-specific Protéine recombinante Ag17358
• Nom complet: aldehyde dehydrogenase 1 family, member A1
• Masse moléculaire calculée: 501 aa, 55 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC001505
• Symbole du gène: ALDH1A1
• Identification du gène (NCBI): 216
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ALDH1A1(Aldehyde dehydrogenase family 1 member A1 ), also named as ALDC, ALDH1 and PUMB1, belongs to the aldehyde dehydrogenase family. The ALDH1A1 gene encodes a liver cytosolic isoform of acetaldehyde dehydrogenase, an enzyme involved in the major pathway of alcohol metabolism after alcohol dehydrogenase. ALDH1A1 plays a critical role in protection against oxidative stress-induced cytotoxicity in lens epithelial cells(PMID:19296407). And it is important for multiple biological activities including drug resistance, cell differentiation, and oxidative stress response(PMID:19025616). As a novel cancer stem cell marker, ALDH1A1 can be used for tumors whose corresponding normal tissues express ALDH1 in relatively restricted or limited levels such as breast, lung, ovarian or colon cancer(PMID: 20422001). This antibody can also recognize some other membranes of the aldehyde dehydrogenase family. This antibody is specific to ALDH1A1.

Publications

Species	Application	Title
human,mouse	WB	Cancer Discov; Transcriptional Silencing of ALDH2 Confers a Dependency on Fanconi Anemia Proteins in Acute Myeloid Leukemia.; Authors - Zhaolin Yang
human	WB	Theranostics; PMID: 28839463; Authors - Junlong Zhuang
human	IP	Commun Biol; PRMT3 interacts with ALDH1A1 and regulates gene-expression by inhibiting retinoic acid signaling.; Authors - Mamta Verma
human	WB	Int J Oncol; Parthenolide inhibits the tumor characteristics of renal cell carcinoma.; Authors - Dandan Liu
human	WB	Neoplasma; Long noncoding RNA ASAP1-IT1 promotes cancer stemness and predicts a poor prognosis in patients with bladder cancer.; Authors - L Yang
human	WB	Oncol Lett; Renal cell carcinoma with synchronous ipsilateral urothelial carcinoma of the renal pelvis.; Authors - Qun Lu