Anticorps Polyclonal de lapin anti-ALDH1A3

• Phare
• Validé par KD/KO

ALDH1A3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(18)

Avis (1)

N° de cat : 25167-1-AP

Synonymes

AL1A3, RALDH-3, EC:1.2.1.36, ALDH6, Aldehyde dehydrogenase 6

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Galerie de données de validation

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  WB analysis using 25167-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25167-1-AP (ALDH1A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 25167-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25167-1-AP (ALDH1A3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 25167-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25167-1-AP (ALDH1A3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 25167-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using ALDH1A3 antibody (25167-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules HepG2, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IHC: tissu hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 16 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Informations sur le produit

25167-1-AP cible ALDH1A3 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Chèvre, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ALDH1A3 Protéine recombinante Ag18605
• Nom complet: aldehyde dehydrogenase 1 family, member A3
• Masse moléculaire calculée: 512 aa, 56 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC069274
• Symbole du gène: ALDH1A3
• Identification du gène (NCBI): 220
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Aldehyde dehydrogenase 1 family member A3 (ALDH1A3), is an aldehyde dehydrogenase enzyme that uses retinal as a substrate either in a free or a cellular retinol-binding protein form. ALDH1A3 plays a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation.ALDH1A3 is calculated to be 56 kDa.

Publications

Species	Application	Title
human	IF	Nature; A human embryonic limb cell atlas resolved in space and time; Authors - Bao Zhang
human	WB	Nat Commun; Decoding non-canonical mRNA decay by the endoplasmic-reticulum stress sensor IRE1α; Authors - Adrien Le Thomas
human	WB	Cell Res; Excessive UBE3A dosage impairs retinoic acid signaling and synaptic plasticity in autism spectrum disorders.; Authors - Xingxing Xu
human	WB	Cell Chem Biol; Lipid-derived electrophiles mediate the effects of chemotherapeutic topoisomerase I poisons.; Authors - Amy Flor
mouse	WB	Mol Metab; Autotaxin signaling facilitates β cell dedifferentiation and dysfunction induced by Sirtuin 3 deficiency.; Authors - Huanyi Cao
human	WB	Free Radic Biol Med; Flavonoid 4,4'-dimethoxychalcone suppresses cell proliferation via dehydrogenase inhibition and oxidative stress aggravation.; Authors - Changmei Yang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Azita (Verified Customer) (06-16-2021)

Immunocytochemistry labelling of (4% PFA) fixed mice spinal cord tissue by ALDH1A3 antibody at dilution of 1:100.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: mice lumbar spinal cord tissue (transverse section 10 µm)

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