Anticorps Polyclonal de lapin anti-ALDH1B1

ALDH1B1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

11

N° de cat : 15560-1-AP

Synonymes

Aldehyde dehydrogenase 5, ALDH1B1, ALDH5, ALDHX

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Galerie de données de validation

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  WB analysis of HepG2 using 15560-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15560-1-AP (ALDH1B1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 15560-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15560-1-AP (ALDH1B1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15560-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15560-1-AP (ALDH1B1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15560-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15560-1-AP (ALDH1B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15560-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15560-1-AP (ALDH1B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 15560-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15560-1-AP (ALDH1B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 15560-1-AP

  IP Result of anti-ALDH1B1 (IP:15560-1-AP, 4ug; Detection:15560-1-AP 1:500) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human testis using 15560-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15560-1-AP (ALDH1B1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 15560-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15560-1-AP (ALDH1B1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 15560-1-AP

  Immunofluorescent analysis of HepG2 cells using 15560-1-AP (ALDH1B1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules K-562, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 3 publications below

Informations sur le produit

15560-1-AP cible ALDH1B1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ALDH1B1 Protéine recombinante Ag7144
• Nom complet: aldehyde dehydrogenase 1 family, member B1
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC001619
• Symbole du gène: ALDH1B1
• Identification du gène (NCBI): 219
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Aldehyde dehydrogenases (ALDHs) belong to a superfamily of NAD(P)+-dependent enzymes, which catalyze the oxidation of endogenous and exogenous aldehydes to their corresponding acids. ALDH1B1 is a mitochondrial ALDH that it is dramatically upregulated in human colonic adenocarcinoma, making it a potential biomarker for human colon cancer.(PMID:21216231). ALDH1B1 is a homotetramer expressed in various adult and fetal human tissues including liver, testis, kidney, skeletal muscle, heart, placenta, brain and lung(PMID:18611112).

Publications

Species	Application	Title
mouse	WB	Mol Cell; Maternal Aldehyde Elimination during Pregnancy Preserves the Fetal Genome.; Authors - Nina Oberbeck
human	WB	Elife; FAK activity sustains intrinsic and acquired ovarian cancer resistance to platinum chemotherapy.; Authors - Carlos J Diaz Osterman
mouse	WB,IHC	J Pathol; Effects of long-term ethanol consumption and Aldh1b1 depletion on intestinal tumourigenesis in mice.; Authors - Mike F Müller
mouse	WB	Biomedicines; β-RA Targets Mitochondrial Metabolism and Adipogenesis, Leading to Therapeutic Benefits against CoQ Deficiency and Age-Related Overweight.; Authors - Agustín Hidalgo-Gutiérrez
mouse	WB	Am J Physiol Regul Integr Comp Physiol; Acetaldehyde dehydrogenase 2 deficiency increases mitochondrial reactive oxygen species emission and induces mitochondrial protease Omi/HtrA2 in skeletal muscle.; Authors - Yuka Wakabayashi
mouse	WB	Am J Physiol Regul Integr Comp Physiol; Aldehyde dehydrogenase 2 deficiency promotes skeletal muscle atrophy in aged mice.; Authors - Akane Kasai