Anticorps Polyclonal de lapin anti-ALDH1B1

ALDH1B1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 22220-1-AP

Synonymes

Aldehyde dehydrogenase 5, Aldehyde dehydrogenase X, mitochondrial, ALDH5, ALDHX, EC:1.2.1.3

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Galerie de données de validation

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  WB analysis of HeLa using 22220-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 22220-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 22220-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 22220-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 22220-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 22220-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 22220-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 22220-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 22220-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 22220-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 22220-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 22220-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22220-1-AP (ALDH1B1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 22220-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 22220-1-AP (ALDH1B1 Antibody) at dilution of 1:50.

• 

  IHC staining of human liver using 22220-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 22220-1-AP (ALDH1B1 Antibody) at dilution of 1:50.

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  IF Staining of MCF-7 using 22220-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ALDH1B1 antibody (22220-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HepG2 using 22220-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ALDH1B1 antibody (22220-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, tissu cérébral de rat, tissu cérébral de souris, tissu testiculaire de souris
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu de cancer du côlon humain, tissu hépatique humain, tissu rénal de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

22220-1-AP cible ALDH1B1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ALDH1B1 Protéine recombinante Ag17557
• Nom complet: aldehyde dehydrogenase 1 family, member B1
• Masse moléculaire calculée: 517 aa, 57 kDa
• Poids moléculaire observé: 55-58 kDa
• Numéro d’acquisition GenBank: BC001619
• Symbole du gène: ALDH1B1
• Identification du gène (NCBI): 219
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Aldehyde dehydrogenases (ALDHs) belong to a superfamily of NAD(P)+-dependent enzymes, which catalyze the oxidation of endogenous and exogenous aldehydes to their corresponding acids. ALDH1B1 is a mitochondrial ALDH that it is dramatically upregulated in human colonic adenocarcinoma, making it a potential biomarker for human colon cancer.(PMID:21216231). ALDH1B1 is a homotetramer expressed in various adult and fetal human tissues including liver, testis, kidney, skeletal muscle, heart, placenta, brain and lung(PMID:18611112). This antibody is specific to ALDH1B1.

Publications

Species	Application	Title
rat	WB	Oncotarget; Proteomics and metabolomics analysis of hepatic mitochondrial metabolism in alcohol-preferring and non-preferring rats.; Authors - Hao-Long Zeng
human	IHC	Transl Cancer Res; ALDH1B1 predicts poor survival for locally advanced nasopharyngeal carcinoma patients.; Authors - Tao Zhu

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