Anticorps Polyclonal de lapin anti-ALDH1L1

ALDH1L1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

Avis (1)

N° de cat : 17390-1-AP

Synonymes

10 FTHFDH, ALDH1L1, FTHFD

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Galerie de données de validation

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  WB analysis using 17390-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17390-1-AP (ALDH1L1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 17390-1-AP

  IP Result of anti-ALDH1L1 (IP:17390-1-AP, 4ug; Detection:17390-1-AP 1:500) with mouse liver tissue lysate 4000ug.

• 

  IP experiment of mouse liver using 17390-1-AP

  IP Result of anti-ALDH1L1 (IP:17390-1-AP, 4ug; Detection:17390-1-AP 1:500) with mouse liver tissue lysate 4000ug.

• 

  IHC staining of human gliomas using 17390-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 17390-1-AP (ALDH1L1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 17390-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 17390-1-AP (ALDH1L1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 17390-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 17390-1-AP (ALDH1L1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 17390-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 17390-1-AP (ALDH1L1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17390-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17390-1-AP (ALDH1L1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17390-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17390-1-AP (ALDH1L1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 17390-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ALDH1L1 antibody (17390-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, foie de rat, foie de souris
• Résultats positifs en IP: tissu hépatique de souris,
• Résultats positifs en IHC: tissu de gliome humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Informations sur le produit

17390-1-AP cible ALDH1L1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ALDH1L1 Protéine recombinante Ag11285
• Nom complet: aldehyde dehydrogenase 1 family, member L1
• Masse moléculaire calculée: 99 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC027241
• Symbole du gène: ALDH1L1
• Identification du gène (NCBI): 10840
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Aldehyde dehydrogenase 1 family member L1 (ALDH1L1), belongs to the aldehyde dehydrogenase family of proteins and is responsible for formate oxidation in vivo. Loss of ALDH1L1 is associated with decreased apoptosis, increased cell motility, and cancer progression. ALDH1L1 catalyzes the conversion of 10-formyltetrahydrofolate, nicotinamide adenine dinucleotide phosphate (NADP+), and water to tetrahydrofolate, NADPH, and carbon dioxide. ALDH1L1 has some isoforms with MW of 99, 88 and 55 kDa.

Publications

Species	Application	Title
mouse	IF	Nature; Reversing a model of Parkinson's disease with in situ converted nigral neurons.; Authors - Hao Qian
human	WB	J Pineal Res; Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.; Authors - Li Cui
mouse	IHC,WB	Nat Commun; Ten-eleven translocation 1 mediated-DNA hydroxymethylation is required for myelination and remyelination in the mouse brain.; Authors - Ming Zhang
human	IHC,WB	NPJ Parkinsons Dis; Reduced astrocytic reactivity in human brains and midbrain organoids with PRKN mutations.; Authors - Masayoshi Kano
mouse	WB,IF	Gene; Immortalization of mouse primary astrocytes; Authors - Weihong Yi
human	IF	Curr Protoc; Primary Astrocytes Purification and Immortalization; Authors - Lei Qin

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Juan (Verified Customer) (01-11-2022)

Working fine

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: hiPSC-derived astrocytes

ALDH1L1 Antibody Immunofluorescence validation (1:50 dilution) in hiPSC-derived astrocytes (Cat no:17390-1-AP)