Anticorps Polyclonal de lapin anti-ALDH2

• Phare
• Validé par KD/KO

ALDH2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

45

Avis (1)

N° de cat : 15310-1-AP

Synonymes

ALDH class 2, ALDH E2, ALDH2, ALDHI, ALDM

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Galerie de données de validation

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  WB analysis using 15310-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15310-1-AP (ALDH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15310-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15310-1-AP (ALDH2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 15310-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 15310-1-AP (ALDH2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 15310-1-AP

  IP result of anti-ALDH2(IP:15310-1-AP, 4ug; Detection:15310-1-AP 1:8000) with HepG2 cells lysate 2040 ug.

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  IHC staining of human liver using 15310-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15310-1-AP (ALDH2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 15310-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15310-1-AP (ALDH2 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of A549 using 15310-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using ALDH2 antibody (15310-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of A549 using 15310-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using ALDH2 antibody (15310-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A549 using 15310-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 15310-1-AP (ALDH2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules HEK-293, cellules HepG2, cellules HuH-7, tissu cardiaque humain, tissu hépatique de rat, tissu rénal de rat, tissu rénal de souris, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IP: cellules HepG2,
• Résultats positifs en IHC: tissu hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 10 publications below
• WB: See 41 publications below
• IHC: See 4 publications below
• IF: See 5 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Informations sur le produit

15310-1-AP cible ALDH2 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ALDH2 Protéine recombinante Ag7452
• Nom complet: aldehyde dehydrogenase 2 family (mitochondrial)
• Masse moléculaire calculée: 56 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC002967
• Symbole du gène: ALDH2
• Identification du gène (NCBI): 217
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Acetaldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. It mediates NADP+-dependent oxidation of aldehydes into acids during detoxification of alcohol-derived acetaldehyde; lipid peroxidation; and metabolism of corticosteroids, biogenic amines and neurotransmitters. Genetic variation in ALDH2 is responsible for individual differences in responses to drinking alcohol. Thus, the absence of this enzyme is linked to alcohol intolerance and a reduced risk for alcoholism-related liver disease.

Publications

Species	Application	Title
human,mouse	WB	Cancer Discov; Transcriptional Silencing of ALDH2 Confers a Dependency on Fanconi Anemia Proteins in Acute Myeloid Leukemia.; Authors - Zhaolin Yang ; KO Validated
human	WB	Blood; Analysis of disease model iPSCs derived from patients with a novel Fanconi anemia-like IBMFS ADH5/ALDH2 deficiency.; Authors - Anfeng Mu ; KO Validated
human,mouse	WB,IHC	Mol Cell; Two Aldehyde Clearance Systems Are Essential to Prevent Lethal Formaldehyde Accumulation in Mice and Humans.; Authors - Felix A Dingler ; KO Validated
mouse	WB	Mol Cell; Maternal Aldehyde Elimination during Pregnancy Preserves the Fetal Genome.; Authors - Nina Oberbeck
human,mouse	WB,IP,IF	J Clin Invest; Acetaldehyde dehydrogenase 2 interactions with LDLR and AMPK regulate foam cell formation.; Authors - Shanshan Zhong ; KO Validated
human,mouse	WB,IF,IP	EMBO J; p53 protects against alcoholic fatty liver disease via ALDH2 inhibition; Authors - Pengbo Yao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hua (Verified Customer) (03-26-2021)

Excellent antibody.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: human liver, mouse liver, mouse brain / HepG2

ALDH2 Antibody Western Blot validation (1:2000 dilution) in human liver, mouse liver, mouse brain / HepG2 (Cat no:15310-1-AP)