Anticorps Monoclonal anti-ALDH2

• Phare
• Validé par KD/KO

ALDH2 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

2G10F4

N° de cat : 68237-1-Ig

Synonymes

ALDH class 2, ALDH E2, ALDH2, ALDHI, ALDM

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Galerie de données de validation

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  WB analysis of mouse liver using 68237-1-Ig

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 68237-1-Ig (ALDH2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig liver using 68237-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 68237-1-Ig (ALDH2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 68237-1-Ig

  WB result of ALDH2 antibody (68237-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALDH2 transfected HepG2 cells.

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  WB analysis of rabbit liver using 68237-1-Ig

  rabbit liver tissue were subjected to SDS PAGE followed by western blot with 68237-1-Ig (ALDH2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 68237-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 68237-1-Ig (ALDH2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, tissu hépatique de lapin, tissu hépatique de rat

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

68237-1-Ig cible ALDH2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ALDH2 Protéine recombinante Ag7463
• Nom complet: aldehyde dehydrogenase 2 family (mitochondrial)
• Masse moléculaire calculée: 56 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC002967
• Symbole du gène: ALDH2
• Identification du gène (NCBI): 217
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Acetaldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. It mediates NADP+-dependent oxidation of aldehydes into acids during detoxification of alcohol-derived acetaldehyde; lipid peroxidation; and metabolism of corticosteroids, biogenic amines and neurotransmitters. Genetic variation in ALDH2 is responsible for individual differences in responses to drinking alcohol. Thus, the absence of this enzyme is linked to alcohol intolerance and a reduced risk for alcoholism-related liver disease.

Publications

Species	Application	Title
mouse	WB	Arch Biochem Biophys; Inhibiting apoptosis and GSDME-mediated pyroptosis attenuates hepatic injury in septic mice; Authors - Na Lu
mouse	WB	Mol Nutr Food Res; Protective Effect and Mechanism of L-Theanine on Acute Alcoholic Liver Injury in Mice; Authors - Meng-Yuan Liu

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