Anticorps Polyclonal de lapin anti-ALDH3A2

ALDH3A2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

6

Avis (1)

N° de cat : 15090-1-AP

Synonymes

Aldehyde dehydrogenase 10, ALDH10, ALDH3A2, FALDH, Fatty aldehyde dehydrogenase, SLS

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Galerie de données de validation

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  WB analysis using 15090-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15090-1-AP (ALDH3A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15090-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15090-1-AP (ALDH3A2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 15090-1-AP

  IP Result of anti-ALDH3A2 (IP:15090-1-AP, 4ug; Detection:15090-1-AP 1:1000) with HEK-293 cells lysate 3000ug.

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  IP experiment of HEK-293 using 15090-1-AP

  IP result of anti-ALDH3A2(IP:15090-1-AP, 4ug; Detection:15090-1-AP 1:6000) with HEK-293 cells lysate 680 ug.

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  IHC staining of human liver cancer using 15090-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15090-1-AP (ALDH3A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15090-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15090-1-AP (ALDH3A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15090-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15090-1-AP (ALDH3A2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15090-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15090-1-AP (ALDH3A2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15090-1-AP

  Immunofluorescent analysis of Hela cells, using ALDH3A2 antibody 15090-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HeLa using 15090-1-AP

  1X10^6 HeLa cells were stained with 0.2ug ALDH3A2 antibody (15090-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 2 publications below

Informations sur le produit

15090-1-AP cible ALDH3A2 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ALDH3A2 Protéine recombinante Ag1687
• Nom complet: aldehyde dehydrogenase 3 family, member A2
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 50-57 kDa
• Numéro d’acquisition GenBank: BC002430
• Symbole du gène: ALDH3A2
• Identification du gène (NCBI): 224
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
rat	IHC	J Cell Mol Med; Taraxacum officinale extract ameliorates dextran sodium sulphate-induced colitis by regulating fatty acid degradation and microbial dysbiosis.; Authors - Wei Chen
	IHC	Cells; Aldehyde Dehydrogenase, a Therapeutic Target in Chordoma: Analysis in 3D Cellular Models.; Authors - Marie-Anaïs Locquet
mouse	WB	J Biol Chem; Long-chain acyl-CoA synthetase 1 interacts with key proteins that activate and direct fatty acids into niche hepatic pathways.; Authors - Pamela A Young
		Front Med (Lausanne); Synthetic antibody-derived immunopeptide provides neuroprotection in glaucoma through molecular interaction with retinal protein histone H3.1; Authors - Kristian Nzogang Fomo
human	WB	Am J Physiol Gastrointest Liver Physiol; Effect of an obesogenic high-fat and high-sucrose diet on hepatic gene expression signatures in male Collaborative Cross mice; Authors - Volodymyr P Tryndyak ; KD Validated
human	WB	Transl Oncol; miR-1182-mediated ALDH3A2 inhibition affects lipid metabolism and progression in ccRCC by activating the PI3K-AKT pathway; Authors - Qingyang Lv ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Elisabeth (Verified Customer) (09-02-2019)

HeLa cells treated with control (Lane 1) or ALDH3A2 (Lane 2) siRNA for 72h.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa

ALDH3A2 Antibody Western Blot, validation (1:1000 dilution) in HeLa (Cat no:15090-1-AP)