Anticorps Polyclonal de lapin anti-ALDH7A1

• Phare
• Validé par KD/KO

ALDH7A1 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

Avis (1)

N° de cat : 10368-1-AP

Synonymes

ALDH7A1, Alpha AASA dehydrogenase, Antiquitin 1, ATQ1, Betaine aldehyde dehydrogenase, EPD, P6c dehydrogenase, PDE

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Galerie de données de validation

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  WB analysis using 10368-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10368-1-AP (ALDH7A1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 10368-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10368-1-AP (ALDH7A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 10368-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10368-1-AP (ALDH7A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 10368-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10368-1-AP (ALDH7A1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 10368-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10368-1-AP (ALDH7A1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HeLa, tissu hépatique de souris, tissu rénal de souris
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

10368-1-AP cible ALDH7A1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ALDH7A1 Protéine recombinante Ag0383
• Nom complet: aldehyde dehydrogenase 7 family, member A1
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC002515
• Symbole du gène: ALDH7A1
• Identification du gène (NCBI): 501
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ALDH7A1(Alpha-aminoadipic semialdehyde dehydrogenase) is also named as ATQ1 and belongs to the aldehyde dehydrogenase family. It plays a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. This protein has 4 isoforms produced by alternative splicing.

Publications

Species	Application	Title
mouse, rat	WB	Arch Toxicol; Protective effects of dioscin against alcohol-induced liver injury.; Authors - Xu Tingting T
rat	WB	Food Funct; Correction: Total saponins from Rosa laevigata Michx fruit attenuates hepatic steatosis induced by high-fat diet in rats.; Authors - Deshi Dong
human	IHC,WB,IF	Exp Cell Res; EPS8 supports pancreatic cancer growth by inhibiting BMI1 mediated proteasomal degradation of ALDH7A1.; Authors - Mingzhu Tan ; KD Validated
rat	WB	PLoS One; Metabolomic mechanisms of gypenoside against liver fibrosis in rats: An integrative analysis of proteomics and metabolomics data.; Authors - Ya-Nan Song
rat	WB	Food Funct; Total saponins from Rosa laevigata Michx fruit attenuates hepatic steatosis induced by high-fat diet in rats.; Authors - Deshi Dong
		J Toxicol Sci; Mechanisms of CCl4-induced liver fibrosis with combined transcriptomic and proteomic analysis.; Authors - Shu Dong

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hua (Verified Customer) (12-04-2018)

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse liver, mouse hippocampus / HepG2

ALDH7A1 Antibody Western Blot, validation (1:1000 dilution) in mouse liver, mouse hippocampus / HepG2 (Cat no:10368-1-AP)