Anticorps Polyclonal de lapin anti-ALDH9A1

ALDH9A1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 26621-1-AP

Synonymes

EC:1.2.1.19, ALDH9, ALDH7, ALDH4, 4-trimethylaminobutyraldehyde dehydrogenase, N-terminally processed

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 26621-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 26621-1-AP (ALDH9A1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 26621-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 26621-1-AP (ALDH9A1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 26621-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26621-1-AP (ALDH9A1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 26621-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26621-1-AP (ALDH9A1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 26621-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26621-1-AP (ALDH9A1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 26621-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26621-1-AP (ALDH9A1 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 26621-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ALDH9A1 antibody (26621-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules HEK-293
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

26621-1-AP cible ALDH9A1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ALDH9A1 Protéine recombinante Ag24312
• Nom complet: aldehyde dehydrogenase 9 family, member A1
• Masse moléculaire calculée: 518 aa, 56 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC151140
• Symbole du gène: ALDH9A1
• Identification du gène (NCBI): 223
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Aldehyde dehydrogenase 9 family member A1(ALDH9A1), belongs to the aldehyde dehydrogenase family of proteins. ALDH9A1 has a high activity for oxidation of gamma-aminobutyraldehyde and other amino aldehydes. ALDH9A1 catalyzes the dehydrogenation of gamma-aminobutyraldehyde to gamma-aminobutyric acid. ALDH9A1 has 3 isoforms with the molecular mass of 46-56 kDa.

Publications

Species	Application	Title
human	WB	Cancers (Basel); Mitochondrial Protein Cox7b Is a Metabolic Sensor Driving Brain-Specific Metastasis of Human Breast Cancer Cells; Authors - Marine C N M Blackman
human	WB	Proteomes; Utilizing Optimized Tools to Investigate PTM Crosstalk: Identifying Potential PTM Crosstalk of Acetylated Mitochondrial Proteins.; Authors - Henrick Horita
human	WB,IHC	Mol Carcinog; C1QBP Promotes Prostate Cancer Progression and Lipid Accumulation by Negatively Regulating ALDH9A1; Authors - Xinyu Liu ; KD Validated

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