Anticorps Polyclonal de lapin anti-ALDOA

• Phare
• Validé par KD/KO

ALDOA Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, RIP, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

44

Avis (1)

N° de cat : 11217-1-AP

Synonymes

ALDA, ALDOA, Aldolase A, Lung cancer antigen NY LU 1, Muscle type aldolase

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Galerie de données de validation

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  WB analysis using 11217-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11217-1-AP (ALDOA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11217-1-AP

  WB result of ALDOA antibody (11217-1-AP; 1:240000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALDOA transfected HeLa cells.

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  WB analysis using 11217-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11217-1-AP (ALDOA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11217-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11217-1-AP (ALDOA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11217-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11217-1-AP (ALDOA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 11217-1-AP

  IP Result of anti-ALDOA (IP:11217-1-AP, 3ug; Detection:11217-1-AP 1:1000) with HepG2 cells lysate 6000ug.

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  IHC staining of human colon cancer using 11217-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11217-1-AP (ALDOA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11217-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11217-1-AP (ALDOA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11217-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11217-1-AP (ALDOA antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 11217-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11217-1-AP (ALDOA antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 11217-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 11217-1-AP (ALDOA antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 11217-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 11217-1-AP (ALDOA antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung cancer using 11217-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11217-1-AP (ALDOA antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 11217-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11217-1-AP (ALDOA antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 11217-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ALDOA antibody (11217-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 11217-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ALDOA antibody (11217-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 11217-1-AP

  1X10^6 HeLa cells were stained with 0.2ug ALDOA antibody (11217-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A549, cellules HeLa, cellules HepG2, cellules Jurkat, cellules MCF-7, cellules NIH/3T3, tissu hépatique de rat
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du poumon humain, tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 39 publications below
• IHC: See 6 publications below
• IF: See 8 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

11217-1-AP cible ALDOA dans les applications de WB, RIP, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ALDOA Protéine recombinante Ag1726
• Nom complet: aldolase A, fructose-bisphosphate
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 35-40 kDa
• Numéro d’acquisition GenBank: BC016800
• Symbole du gène: ALDOA
• Identification du gène (NCBI): 226
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ALDOA(Fructose-bisphosphate aldolase A) is also named as lung cancer antigen NY-LU-1,muscle-type aldolase,ALDA and belongs to the class I fructose-bisphosphate aldolase family.The ALDOA gene encodes a homotetrameric protein(PMID:14615364).It is found in skeletal muscle and red blood cells, and functions in glycolysis, promoting the reversible cleavage of FBP to triose phosphate, G-3-P and DHAP. ALDOA is upregulated in various cancers, including human lung squamous and renal cell carcinomas(PMID:19937139).

Publications

Species	Application	Title
human	WB	Cancer Cell; Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.; Authors - Ling Li
mouse	WB	Nat Neurosci; Metabolic lactate production coordinates vasculature development and progenitor behavior in the developing mouse neocortex.; Authors - Xiaoxiang Dong
mouse	WB	Dev Cell; Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development.; Authors - Vinay Bulusu
human,mouse	WB	Int J Biol Sci; Chronic stress promotes colorectal cancer progression by enhancing glycolysis through β2-AR/CREB1 signal pathway; Authors - Yunfeng Guan
human	IF	Mol Ther; Single-Cell Transcriptome Analysis Reveals Intratumoral Heterogeneity in ccRCC, which Results in Different Clinical Outcomes.; Authors - Junyi Hu
mouse	WB	eLife; Glycolytic flux-signaling controls mouse embryo mesoderm development; Authors - Hidenobu Miyazawa

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

K (Verified Customer) (12-20-2020)

Worked well for IHC at 1:200 dilution

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: mouse liver tissues and rat liver