Anticorps Recombinant de lapin anti-ALDOA

• Phare
• Validé par KD/KO

ALDOA Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

2F4

N° de cat : 80837-1-RR

Synonymes

Lung cancer antigen NY-LU-1, Fructose-bisphosphate aldolase A, EC:4.1.2.13, Aldolase A, ALDA

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Galerie de données de validation

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  WB analysis using 80837-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80837-1-RR (ALDOA antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 80837-1-RR

  WB result of ALDOA antibody (80837-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALDOA transfected HEK-293 cells.

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  IF Staining of HeLa using 80837-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ALDOA antibody (80837-1-RR, Clone: 2F4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of HeLa using 80837-1-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ALDOA antibody (80837-1-RR, Clone: 2F4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  Affinity and Kinetic Characterization of 80837-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80837-1-RR against Human ALDOA were performed. The affinity constant is 1.34 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules 4T1, cellules HEK-293, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules NIH/3T3, tissu de muscle squelettique de porc, tissu hépatique de porc
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

80837-1-RR cible ALDOA dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ALDOA Protéine recombinante Ag1726
• Nom complet: aldolase A, fructose-bisphosphate
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 35-40 kDa
• Numéro d’acquisition GenBank: BC016800
• Symbole du gène: ALDOA
• Identification du gène (NCBI): 226
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ALDOA(Fructose-bisphosphate aldolase A) is also named as lung cancer antigen NY-LU-1,muscle-type aldolase,ALDA and belongs to the class I fructose-bisphosphate aldolase family.The ALDOA gene encodes a homotetrameric protein(PMID:14615364).It is found in skeletal muscle and red blood cells, and functions in glycolysis, promoting the reversible cleavage of FBP to triose phosphate, G-3-P and DHAP. ALDOA is upregulated in various cancers, including human lung squamous and renal cell carcinomas(PMID:19937139).

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