Anticorps Polyclonal de lapin anti-ALDOB

• Phare
• Validé par KD/KO

ALDOB Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

30

N° de cat : 18065-1-AP

Synonymes

ALDB, ALDOB, Liver type aldolase

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Galerie de données de validation

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  WB analysis using 18065-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18065-1-AP (ALDOB antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 18065-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18065-1-AP (ALDOB antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 18065-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18065-1-AP (ALDOB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 18065-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18065-1-AP (ALDOB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 18065-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 18065-1-AP (ALDOB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 18065-1-AP

  IP result of anti-ALDOB (IP:18065-1-AP, 4ug; Detection:18065-1-AP 1:500) with mouse brain tissue lysate 2400 ug.

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  IP experiment of mouse kidney using 18065-1-AP

  IP Result of anti-ALDOB (IP:18065-1-AP, 4ug; Detection:18065-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human liver cancer using 18065-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18065-1-AP (ALDOB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 18065-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18065-1-AP (ALDOB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules Raji, tissu cérébral de souris, tissu de muscle squelettique de souris, tissu hépatique de souris, tissu rénal de rat
• Résultats positifs en IP: tissu cérébral de souris, tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 27 publications below
• IHC: See 5 publications below
• IF: See 5 publications below
• IP: See 2 publications below

Informations sur le produit

18065-1-AP cible ALDOB dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ALDOB Protéine recombinante Ag12695
• Nom complet: aldolase B, fructose-bisphosphate
• Masse moléculaire calculée: 316 aa, 35 kDa
• Poids moléculaire observé: 35-40 kDa
• Numéro d’acquisition GenBank: BC029399
• Symbole du gène: ALDOB
• Identification du gène (NCBI): 229
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fructose-1,6-bisphosphate aldolase is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate.Vertebrates have 3 aldolase isozymes, aldolase A (ALDOA), B (ALDOB), and C (ALDOC). Deficiency of this enzyme ALDOB causes an accumulation of fructose-1-phosphate after fructose intake, which results in toxic symptoms like vomiting, hypoglycemia, jaundice, elevated liver enzymes and hepatomegaly(PMID:22375183). This antibody may detect ALDOA.

Publications

Species	Application	Title
mouse	WB	Nature; Fructose-1,6-bisphosphate and aldolase mediate glucose sensing by AMPK.; Authors - Chen-Song Zhang ; KD Validated
human	IF	Cancer Cell; Proteogenomic characterization identifies clinically relevant subgroups of intrahepatic cholangiocarcinoma.; Authors - Liangqing Dong
human	WB	Nat Commun; O-GlcNAc modification of leucyl-tRNA synthetase 1 integrates leucine and glucose availability to regulate mTORC1 and the metabolic fate of leucine.; Authors - Kibum Kim
human	WB	Cell Res; cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.; Authors - Linchong Sun
human	IF	Mol Ther; Single-Cell Transcriptome Analysis Reveals Intratumoral Heterogeneity in ccRCC, which Results in Different Clinical Outcomes.; Authors - Junyi Hu
human	WB,IP	Free Radic Biol Med; SIRT5-mediated SDHA desuccinylation promotes clear cell renal cell carcinoma tumorigenesis.; Authors - Yuanzhen Ma