Anticorps Monoclonal anti-Aldolase C

Aldolase C Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

5H7A5

N° de cat : 66120-1-Ig

Synonymes

ALDOC, 5H7A5, ALDC, ALDO, Brain-type aldolase

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Galerie de données de validation

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  WB analysis using 66120-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66120-1-Ig (Aldolase C antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis of human heart using 66120-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 66120-1-Ig (Aldolase C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 66120-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66120-1-Ig (Aldolase C antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 66120-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66120-1-Ig (Aldolase C antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66120-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66120-1-Ig (Aldolase C antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 66120-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66120-1-Ig (Aldolase C antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 66120-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66120-1-Ig (Aldolase C antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66120-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66120-1-Ig (Aldolase C antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66120-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66120-1-Ig (Aldolase C antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66120-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Aldolase C antibody (66120-1-Ig, Clone: 5H7A5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  IF Staining of HepG2 using 66120-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Aldolase C antibody (66120-1-Ig, Clone: 5H7A5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules HSC-T6, cellules K-562, tissu cardiaque humain, tissu cérébral de porc, tissu cérébral de souris, tissu cérébral humain, tissu de cervelet de rat, tissu de cervelet de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu cérébral de souris, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66120-1-Ig cible Aldolase C dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Aldolase C Protéine recombinante Ag6779
• Nom complet: aldolase C, fructose-bisphosphate
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC003613
• Symbole du gène: Aldolase C
• Identification du gène (NCBI): 230
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fructose-bisphosphate aldolase C (ALDOC) reversibly cleaves FBP and F1-P to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate and is strongly expressed in mammalian brain together with ALDOA and is alsopresent in the heart and spleen of some species(PMID:9363598). It is involed in glycolysis as an important enzyme. Phospholipase D2 and inositol 1,4,5-triphosphate interact with ALDOC in signal transduction. Meanwhile, the protein expression of ALDOC has been reported to be regulated in brain tumor, hepatomas, and lung cancer(PMID:21548097).

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