Anticorps Polyclonal de lapin anti-ALG13

• Phare
• Validé par KD/KO

ALG13 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications(3)

Avis (1)

N° de cat : 20810-1-AP

Synonymes

ALG13, CXorf45, GLT28D1, MDS031, YGL047W

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Galerie de données de validation

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  WB analysis of HepG2 using 20810-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20810-1-AP (ALG13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 20810-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20810-1-AP (ALG13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 20810-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20810-1-AP (ALG13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 20810-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 20810-1-AP (ALG13 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human stomach using 20810-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 20810-1-AP (ALG13 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules Jurkat, cellules K-562
• Résultats positifs en IHC: tissu d'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

20810-1-AP cible ALG13 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ALG13 Protéine recombinante Ag14763
• Nom complet: asparagine-linked glycosylation 13 homolog
• Masse moléculaire calculée: 1137 aa, 126 kDa
• Poids moléculaire observé: 130-135 kDa
• Numéro d’acquisition GenBank: BC005336
• Symbole du gène: ALG13
• Identification du gène (NCBI): 79868
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	J Inherit Metab Dis; Predominant and novel de novo variants in 29 individuals with ALG13 deficiency: Clinical description, biomarker status, biochemical analysis, and treatment suggestions.; Authors - Bobby G Ng
mouse	IF	Nephron; Dysregulation of the Expression of Asparagine-Linked Glycosylation 13 Short Isoform 2 Affects Nephrin Function by Altering Its N-Linked Glycosylation.; Authors - Teresa Esposito
mouse	WB,IHC,IF	Neuroscience; ALG13 Deficiency Associated with Increased Seizure Susceptibility and Severity.; Authors - Peng Gao ; KO Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kathryn (Verified Customer) (07-09-2020)

Works beautifully on cryopreserved ovarian tissue. Image provided is postnatal day 4 ovary stained with anti-ALG13 in red and anti-TRA98 in green.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: neonatal ovary

ALG13 Antibody Immunofluorescence, validation (1:100 dilution) in neonatal ovary (Cat no:20810-1-AP)

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