Anticorps Polyclonal de lapin anti-ALKBH4

• Phare
• Validé par KD/KO

ALKBH4 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 19882-1-AP

Synonymes

ABH4, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 4, DNA N6-methyl adenine demethylase ALKBH4, EC:1.14.11.-, EC:1.14.11.51

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Galerie de données de validation

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  WB analysis of mouse kidney using 19882-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19882-1-AP (ALKBH4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 19882-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 19882-1-AP (ALKBH4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 19882-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 19882-1-AP (ALKBH4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 19882-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 19882-1-AP (ALKBH4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 19882-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ALKBH4 antibody (19882-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules K-562, cellules MCF-7, tissu ovarien de souris
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IF: See 1 publications below

Informations sur le produit

19882-1-AP cible ALKBH4 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ALKBH4 Protéine recombinante Ag8041
• Nom complet: alkB, alkylation repair homolog 4 (E. coli)
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC002820
• Symbole du gène: ALKBH4
• Identification du gène (NCBI): 54784
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Cancer Discov; Identification of BBOX1 as a Therapeutic Target in Triple-Negative Breast Cancer.; Authors - Chengheng Liao ; KD Validated
human	WB,IF	Sci Rep; ALKBH overexpression in head and neck cancer: potential target for novel anticancer therapy.; Authors - Tomaš Pilžys ; KD Validated
human	WB	Acta Biochim Pol; Selected ALKBH dioxygenases are overexpressed in salivary gland tumours; Authors - Michał Marcinkowski
mouse,human	WB	Water (Basel); ALKBH4 Stabilization Is Required for Arsenic-Induced 6mA DNA Methylation Inhibition, Keratinocyte Malignant Transformation, and Tumorigenicity; Authors - Yan-Hong Cui ; KD Validated
pig	WB	Sci Rep; The proteomic profile is altered but not repaired after bariatric surgery in type 2 diabetes pigs; Authors - Karolina Ferenc

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