Anticorps Polyclonal de lapin anti-PLAP

• Phare
• Validé par KD/KO

PLAP Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, FC, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 18507-1-AP

Synonymes

ALP, ALPP, PALP, Placental alkaline phosphatase, PLAP, PLAP 1

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Galerie de données de validation

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  WB analysis of human placenta using 18507-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 18507-1-AP (PLAP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 18507-1-AP

  WB result of PLAP antibody (18507-1-AP, 1:500) with si-control and si-PLAP transfected HepG2 cells.

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  WB analysis of mouse liver using 18507-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18507-1-AP (PLAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 18507-1-AP

  IP Result of anti-PLAP (IP:18507-1-AP, 4ug; Detection:18507-1-AP 1:700) with HepG2 cells lysate 1800ug.

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  IHC staining of human placenta using 18507-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18507-1-AP (PLAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 18507-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18507-1-AP (PLAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 18507-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18507-1-AP (PLAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HepG2 using 18507-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human PLAP (18507-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu placentaire humain, cellules HepG2, tissu hépatique de souris
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu placentaire humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IHC: See 1 publications below

Informations sur le produit

18507-1-AP cible PLAP dans les applications de WB, IP, IHC, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PLAP Protéine recombinante Ag13361
• Nom complet: alkaline phosphatase, placental (Regan isozyme)
• Masse moléculaire calculée: 58 kDa
• Poids moléculaire observé: 66 kDa
• Numéro d’acquisition GenBank: BC009647
• Symbole du gène: ALPP
• Identification du gène (NCBI): 250
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Placental alkaline phosphatase (PLAP) is a glycosyl phosphatidylinositol (GPI)-anchored sialoglycoprotein , which shows greater resistance to heat inactivation than other alkaline phosphatase. Presented at high levels in placental trophoblasts, PLAP is recognized as an important marker of differentiation in human malignancies.

Publications

Species	Application	Title
mouse	WB	Aging (Albany NY); Primary cilia regulate gastric cancer-induced bone loss via cilia/Wnt/β-catenin signaling pathway.; Authors - Jie Xu
human	IHC	ACS Biomater Sci Eng; 6-Bromoindirubin-3'-oxime Promotes Osteogenic Differentiation of Periodontal Ligament Stem Cells and Facilitates Bone Regeneration in a Mouse Periodontitis Model.; Authors - Song Shen
mouse	WB	Mol Cell Endocrinol; Long noncoding RNA ZFAS1 suppresses osteogenic differentiation of bone marrow-derived mesenchymal stem cells by upregulating miR-499-EPHA5 axis.; Authors - Jinhui Wu
	WB	J Dent; PMID: 32119967; Authors - Yunxia Zhang
human	WB	Amino Acids; New aspects of antiproliferative activity of 4-hydroxybenzyl isothiocyanate, a natural H2S-donor.; Authors - Halina Jurkowska
mouse	WB	Biomaterials; NRF2 is a critical regulator and therapeutic target of metal implant particle-incurred bone damage; Authors - Jian Dong

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C