Anticorps Polyclonal de lapin anti-AMH

AMH Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 14461-1-AP

Synonymes

AMH, anti Mullerian hormone, MIF, MIS

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Galerie de données de validation

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  WB analysis of human testis using 14461-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 14461-1-AP (AMH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse ovary using 14461-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 14461-1-AP (AMH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 14461-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 14461-1-AP (AMH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat ovary using 14461-1-AP

  Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 14461-1-AP (AMH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat ovary using 14461-1-AP

  Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 14461-1-AP (AMH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 14461-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 14461-1-AP (AMH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 14461-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 14461-1-AP (AMH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 14461-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 14461-1-AP (AMH antibody) at dilution of 1:25 (under 10x lens).

• 

  IHC staining of human ovary tumor using 14461-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 14461-1-AP (AMH antibody) at dilution of 1:25 (under 40x lens).

• 

  IHC staining of human testis using 14461-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14461-1-AP (AMH antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 14461-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14461-1-AP (AMH antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 14461-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14461-1-AP (AMH antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 14461-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14461-1-AP (AMH antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of mouse ovary using 14461-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse ovary tissue using AMH antibody (14461-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse ovary using 14461-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse ovary tissue using AMH antibody (14461-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu testiculaire humain,
• Résultats positifs en IHC: tissu ovarien de souris, tissu de tumeur ovarienne humain, tissu ovarien de rat, tissu pancréatique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu ovarien de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Informations sur le produit

14461-1-AP cible AMH dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Chèvre, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AMH Protéine recombinante Ag5670
• Nom complet: anti-Mullerian hormone
• Masse moléculaire calculée: 59 kDa
• Numéro d’acquisition GenBank: BC049194
• Symbole du gène: AMH
• Identification du gène (NCBI): 268
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Anti-Müllerian hormone (AMH), also called Müllerian-inhibiting substance (MIS), is a hormone that is best known for its production by fetal testes in mammals and as the inhibitor of Müllerian duct development in males. AMH is also expressed in granulosa cells of preantral and small antral follicles in the ovary. More recently, AMH has been studied for its role in ovarian folliculogenesis and as a potential marker of ovarian reserve. The deduced protein sequence of the AMH monomer contains a 25 amino acid secretion specific signal peptide and a monomeric protein of 535 amino acids that, when glycosylated at two glycosylation sites, has a molecular weight of 65-70 kDa. This antibody is specific to AMH.

Publications

Species	Application	Title
chicken	WB	Theriogenology; miR-140-3p promotes follicle granulosa cell proliferation and steroid hormone synthesis via targeting AMH in chickens; Authors - Bei-Bei Zhang ; KD Validated
mouse	WB	Exp Cell Res; Proteomic analysis of mouse ovaries during the prepubertal stages.; Authors - Jiaqiang Xiong
		Hum Reprod; Peroxisome proliferator-activated receptor-γ coactivator 1α-mediated pathway as a possible therapeutic target in endometriosis.; Authors - Hisashi Kataoka
mouse	IHC	J Funct Biomater; Construction of Artificial Ovaries with Decellularized Porcine Scaffold and Its Elicited Immune Response after Xenotransplantation in Mice; Authors - Tong Wu
rat	WB	Basic Clin Pharmacol Toxicol; Effects of quercetin on ovarian function and regulation of the ovarian PI3K/Akt/FoxO3a signalling pathway and oxidative stress in a rat model of cyclophosphamide-induced premature ovarian failure; Authors - Shaoyan Zheng
goat	WB	Theriogenology; BMP6 regulates AMH expression via SMAD1/5/8 in goat ovarian granulosa cells; Authors - Lei Wang