Anticorps Monoclonal anti-AMOTL2

AMOTL2 Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

2C4A4

N° de cat : 68401-1-Ig

Synonymes

AMOTL2, angiomotin like 2, Angiomotin like protein 2, KIAA0989, LCCP, Leman coiled coil protein

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Galerie de données de validation

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  WB analysis using 68401-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68401-1-Ig (AMOTL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of DU 145 using 68401-1-Ig

  DU 145 cells were subjected to SDS PAGE followed by western blot with 68401-1-Ig (AMOTL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Saos-2 using 68401-1-Ig

  Saos-2 cells were subjected to SDS PAGE followed by western blot with 68401-1-Ig (AMOTL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of NCI-H1299 using 68401-1-Ig

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 68401-1-Ig (AMOTL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of A549 using 68401-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using AMOTL2 antibody (68401-1-Ig, Clone: 2C4A4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules DU 145, cellules MDA-MB-468, cellules NCI-H1299
• Résultats positifs en IF/ICC: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68401-1-Ig cible AMOTL2 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: AMOTL2 Protéine recombinante Ag19971
• Nom complet: angiomotin like 2
• Masse moléculaire calculée: 779 aa, 86 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC011454
• Symbole du gène: AMOTL2
• Identification du gène (NCBI): 51421
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Angiomotin‑like 2 (AMOTL2) is a member of the motin family of angiostatin‑binding proteins. The motin family, also known as AMOTs, consists of three members: AMOT, AMOT-like 1 (AMOTL1) and AMOTL2. Members of the motin family are a type of adaptor proteins mainly distributed in the cytomembrane, cytoplasm or nucleus, and have a higher expression in the endothelial cells of capillaries and in larger vessels of the placenta. Human AmotL2 encodes two isoforms of a molecular mass of 100 kDa and 60 kDa.(PMID: 34036399,PMID: 25080976)

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