Anticorps Polyclonal de lapin anti-AMPD3

• Phare
• Validé par KD/KO

AMPD3 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 23997-1-AP

Synonymes

AMPD 3, EC:3.5.4.6

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Galerie de données de validation

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  WB analysis using 23997-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23997-1-AP (AMPD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 23997-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 23997-1-AP (AMPD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 23997-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 23997-1-AP (AMPD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat kidney using 23997-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 23997-1-AP (AMPD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 23997-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23997-1-AP (AMPD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 23997-1-AP

  IP result of anti-AMPD3 (IP:23997-1-AP, 4ug; Detection:23997-1-AP 1:800) with mouse kidney tissue lysate 4800ug.

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  IHC staining of human colon using 23997-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 23997-1-AP (AMPD3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse placenta using 23997-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 23997-1-AP (AMPD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 23997-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 23997-1-AP (AMPD3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 23997-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 23997-1-AP (AMPD3 Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules RAW 264.7, cellules HEK-293, tissu pulmonaire de souris, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IP: tissu rénal de souris
• Résultats positifs en IHC: tissu de côlon humain, tissu placentaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

23997-1-AP cible AMPD3 dans les applications de WB, IHC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AMPD3 Protéine recombinante Ag21199
• Nom complet: adenosine monophosphate deaminase (isoform E)
• Masse moléculaire calculée: 776 aa, 90 kDa
• Poids moléculaire observé: 70 kDa, 90 kDa
• Numéro d’acquisition GenBank: BC126118
• Symbole du gène: AMPD3
• Identification du gène (NCBI): 272
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
rat	WB,IP	J Mol Cell Cardiol; Translational regulation by miR-301b upregulates AMP deaminase in diabetic hearts.; Authors - Yuki Tatekoshi
		Mol Cell Biochem; Role of AMP deaminase in diabetic cardiomyopathy; Authors - Tetsuji Miura
rat	WB	J Mol Cell Cardiol; Xanthine oxidoreductase-mediated injury is amplified by upregulated AMP deaminase in type 2 diabetic rat hearts under the condition of pressure overload.; Authors - Yusuke Igaki
mouse,human	WB,IHC	Nat Metab; Super-enhancer-driven metabolic reprogramming promotes cystogenesis in autosomal dominant polycystic kidney disease.; Authors - Zeyun Mi ; KD Validated
mouse	WB	J Med Chem; Discovery of Novel N-(5-(Pyridin-3-yl)-1H-indazol-3-yl)benzamide Derivatives as Potent Cyclin-Dependent Kinase 7 Inhibitors for the Treatment of Autosomal Dominant Polycystic Kidney Disease; Authors - Bowen Yang
rat	WB,IP	Physiol Rep; Downregulation of extramitochondrial BCKDH and its uncoupling from AMP deaminase in type 2 diabetic OLETF rat hearts; Authors - Toshifumi Ogawa

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