Anticorps Monoclonal anti-Pancreatic alpha-amylase

Pancreatic alpha-amylase Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(4)

CloneNo.

2F1G8

N° de cat : 66133-1-Ig

Synonymes

AMY2A, Amylase Alpha, Amylase, alpha, AMY2B, AMY2

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Galerie de données de validation

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  WB analysis of human saliva using 66133-1-Ig

  human saliva were subjected to SDS PAGE followed by western blot with 66133-1-Ig (Amylase, alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human saliva using 66133-1-Ig

  0.1 μL human saliva lysate were subjected to SDS PAGE followed by western blot with 66133-1-Ig (Amylase, alpha antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig pancreas using 66133-1-Ig

  0.6 μg pig pancreas tissue lysate were subjected to SDS PAGE followed by western blot with 66133-1-Ig (Amylase, alpha antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat pancreas using 66133-1-Ig

  0.6 μg rat pancreas tissue lysate were subjected to SDS PAGE followed by western blot with 66133-1-Ig (Amylase, alpha antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 66133-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66133-1-Ig (Amylase, alpha antibody at dilution of 1:50(under 10x lens).

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  IHC staining of human pancreas cancer using 66133-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66133-1-Ig (Amylase, alpha antibody at dilution of 1:50(under 40x lens).

• 

  IF Staining of BxPC-3 using 66133-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using Amylase Alpha antibody (66133-1-Ig, Clone: 2F1G8 ) at dilution of 1:1200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

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Applications testées

• Résultats positifs en WB: salive humain, tissu, tissu pancréatique de porc, tissu pancréatique de rat
• Résultats positifs en IHC: tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules BxPC-3,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:600-1:2400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

66133-1-Ig cible Pancreatic alpha-amylase dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat

• Réactivité: Humain, porc, rat
• Réactivité citée: Humain, porc
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Pancreatic alpha-amylase Protéine recombinante Ag8819
• Nom complet: amylase, alpha 2A (pancreatic)
• Masse moléculaire calculée: 511 aa, 58 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: BC007060
• Symbole du gène: Amylase Alpha
• Identification du gène (NCBI): 279
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The complete human amylase gene contains five intact genes: two pancreatic genes (AMY2A and AMY2B) and three salivary genes (AMYIA, AMYIB, and AMYIC). AMY2A(Pancreatic alpha-amylase)is also named as PA and is a 56 kDa protein consisting of 496 amino acids in a single polypeptide chain, folded into three domains(PMID:10657258). It has a hydrolase activity acting on glycosyl bonds. AMY2A and AMY2B transcripts are present in the human pancreas but not in the parotid salivary gland, while AMY1 transcripts are present in the parotid gland but not in the pancreas(PMID:1692956).This antibody can recognize amylase alpha.

Publications

Species	Application	Title
	IF	Am J Physiol Gastrointest Liver Physiol; Is the intestinal mucous layer a NAtural Deep Eutectic Solvent (NADES) based digestion matrix; Authors - Theo A T G van Kempen
human	IHC	J Toxicol Pathol; Pancreatic Ductal Adenocarcinoma in a Wistar Hannover GALAS Rat.; Authors - Yusuke Kuroda
pig	IHC	Biochem Biophys Rep; Multiple cystic sphere formation from PK-8 cells in three-dimensional culture; Authors - Yuuki Shichi
human	WB	J Proteome Res; Proteomic Analysis of Differences in the Freezability of Porcine Sperm Identifies α-Amylase As a Key Protein; Authors - Kejun Wang

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