Anticorps Polyclonal de lapin anti-APC1

APC1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 21748-1-AP

Synonymes

TSG 24, Testis-specific gene 24 protein, Mitotic checkpoint regulator, Apc 1, Anaphase-promoting complex subunit 1

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Galerie de données de validation

• 

  WB analysis of HeLa using 21748-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21748-1-AP (APC1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Transfected HEK-293 using 21748-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 21748-1-AP (ANAPC1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 21748-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 21748-1-AP (APC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 21748-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 21748-1-AP (APC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 21748-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21748-1-AP (APC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 21748-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21748-1-AP (APC1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 21748-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21748-1-AP (APC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 21748-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21748-1-AP (APC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 21748-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 21748-1-AP (APC1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human cervical cancer using 21748-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 21748-1-AP (APC1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human stomach using 21748-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 21748-1-AP (APC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach using 21748-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 21748-1-AP (APC1 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HEK-293 transfectées, cellules HT-1080, cellules K-562, tissu cérébral de souris
• Résultats positifs en IHC: tissu testiculaire de souris, tissu de cancer du col de l'utérus humain, tissu d'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 1 publications below

Informations sur le produit

21748-1-AP cible APC1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: APC1 Protéine recombinante Ag13399
• Nom complet: anaphase promoting complex subunit 1
• Masse moléculaire calculée: 1944 aa, 217 kDa
• Poids moléculaire observé: 200-210 kDa
• Numéro d’acquisition GenBank: BC104902
• Symbole du gène: APC1
• Identification du gène (NCBI): 64682
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Nat Commun; DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation; Authors - Xiaohua Xu
human	WB	Oncotarget; Screening key microRNAs for castration-resistant prostate cancer based on miRNA/mRNA functional synergistic network.; Authors - Jin Zhu
human	WB	Sci Rep; Reciprocal interaction between SIRT6 and APC/C regulates genomic stability.; Authors - Helin Wang
human	WB	Cell Rep; TRPS1 drives heterochromatic origin refiring and cancer genome evolution.; Authors - Jianguo Yang
human	WB	Cell Biosci; PATL2 mutations affect human oocyte maternal mRNA homeostasis and protein interactions in cell cycle regulation; Authors - Yin-Li Zhang
human	WB,IHC	Cancer Control; The Potential Biological Roles and Clinical Significance of Anaphase-Promoting Complex Subunit 1 in Colorectal Cancer; Authors - Yi Chen

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