Anticorps Recombinant de lapin anti-ANK2

ANK2 Recombinant Antibody for IHC, IF-P, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

IHC, IF-P, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240982G5

N° de cat : 83912-5-RR

Synonymes

Ankyrin 2, ANK 2, 240982G5

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Galerie de données de validation

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  IHC staining of mouse cerebellum using 83912-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83912-5-RR (ANK2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 83912-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83912-5-RR (ANK2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse heart using 83912-5-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using ANK2 antibody (83912-5-RR, Clone: 240982G5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse heart using 83912-5-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using ANK2 antibody (83912-5-RR, Clone: 240982G5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HEK-293 using 83912-5-RR

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug Ank2 Recombinant Antibody (83912-5-RR, Clone:240982G5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83912-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83912-5-RR against Human ANK2 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en IHC: tissu de cervelet de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cardiaque de souris,
• Résultats positifs en FC (Intra): cellules HEK-293,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83912-5-RR cible ANK2 dans les applications de IHC, IF-P, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ANK2 Protéine recombinante Ag34642
• Nom complet: ankyrin 2, neuronal
• Masse moléculaire calculée: 434 kDa
• Poids moléculaire observé: 250-260 kDa
• Numéro d’acquisition GenBank: NM_001148
• Symbole du gène: ANK2
• Identification du gène (NCBI): 287
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The Ankyrins, belonging to the adapter protein family, consist of Ankyrin-R (ANK1), Ankyrin-B (ANK2), and Ankyrin-G (ANK3). Initially, ANK2 was considered as a key cytoskeletal-associated protein in the brain. Besides, it was discovered as a pivotal adaptor and scaffolding protein in the heart. Remarkably, recent studies have demonstrated that Ankyrins contribute to the modulation of various biological activities in cancers. (PMID: 36539782)

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