Anticorps Polyclonal de lapin anti-ANKRD6

• Phare
• Validé par KD/KO

ANKRD6 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 24333-1-AP

Synonymes

Diversin, Ankyrin repeat domain-containing protein 6, ANKRD 6

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Galerie de données de validation

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  WB analysis of HEK-293 using 24333-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24333-1-AP (ANKRD6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 24333-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 24333-1-AP (ANKRD6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 24333-1-AP

  IP result of anti-ANKRD6 (IP:24333-1-AP, 5ug; Detection:24333-1-AP 1:300) with HEK-293 cells lysate 920ug.

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  IHC staining of mouse ovary using 24333-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 24333-1-AP (ANKRD6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 24333-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 24333-1-AP (ANKRD6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 24333-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24333-1-AP (ANKRD6 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 24333-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24333-1-AP (ANKRD6 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 24333-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 24333-1-AP (ANKRD6 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 24333-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 24333-1-AP (ANKRD6 Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, tissu cérébral humain fœtal
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu ovarien de souris, tissu ovarien humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below

Informations sur le produit

24333-1-AP cible ANKRD6 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ANKRD6 Protéine recombinante Ag19328
• Nom complet: ankyrin repeat domain 6
• Masse moléculaire calculée: 727 aa, 80 kDa
• Poids moléculaire observé: 80-90 kDa
• Numéro d’acquisition GenBank: BC042173
• Symbole du gène: ANKRD6
• Identification du gène (NCBI): 22881
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ankrd6 (Ankyrin repeat domain 6) is a distant homolog of the fly PCP mediator diego and acts as a molecular switch between both Wnt pathways. It promotes signaling of the non canonical Wnt/PCP pathway and simultaneously inhibits gene transcription through the canonical Wnt/β-catenin pathway.

Publications

Species	Application	Title
human	IHC	Chin J Cancer Res; Pan-cancer analyses demonstrate that ANKRD6 is associated with a poor prognosis and correlates with M2 macrophage infiltration in colon cancer.; Authors - Rui Bai
human	WB	Mol Carcinog; Role of miR-214 in regulation of β-catenin and the malignant phenotype of melanoma.; Authors - Kirthana Prabhakar ; KD Validated
human	IHC	Biomedicines; The Roles of AGTRAP, ALKBH3, DIVERSIN, NEDD8 and RRM1 in Glioblastoma Pathophysiology and Prognosis; Authors - Claudia Alexandra Dumitru
rat	WB	Reprod Biol Endocrinol; Map-1a regulates Sertoli cell BTB dynamics through the cytoskeletal organization of microtubule and F-actin; Authors - Lingling Wang
human	WB	Endocrinology; The planar cell polarity (PCP) protein Fat 1 in Sertoli cell function; Authors - Tiao Bu

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