Anticorps Polyclonal de lapin anti-ANTXR2

• Phare
• Validé par KD/KO

ANTXR2 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(9)

N° de cat : 16723-1-AP

Synonymes

anthrax toxin receptor 2, ANTXR2, CMG 2, CMG2, ISH, JHF

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Galerie de données de validation

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  WB analysis of HEK-293 using 16723-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16723-1-AP (ANTXR2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 16723-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16723-1-AP (ANTXR2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 16723-1-AP

  IP result of anti-ANTXR2 (IP:16723-1-AP, 4ug; Detection:16723-1-AP 1:500) with HEK-293 cells lysate 2800ug.

• 

  IHC staining of human ovary using 16723-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 16723-1-AP (ANTXR2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human ovary using 16723-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 16723-1-AP (ANTXR2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human kidney using 16723-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16723-1-AP (ANTXR2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 16723-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16723-1-AP (ANTXR2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 16723-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16723-1-AP (ANTXR2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 16723-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16723-1-AP (ANTXR2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 16723-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16723-1-AP (ANTXR2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 16723-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16723-1-AP (ANTXR2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 16723-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16723-1-AP (ANTXR2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 16723-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16723-1-AP (ANTXR2 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules PC-3
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu ovarien humain, tissu placentaire humain, tissu pulmonaire humain, tissu rénal humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 4 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

16723-1-AP cible ANTXR2 dans les applications de WB, IHC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ANTXR2 Protéine recombinante Ag10162
• Nom complet: anthrax toxin receptor 2
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC107876
• Symbole du gène: ANTXR2
• Identification du gène (NCBI): 118429
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ANTXR2 (anthrax toxin receptor 2), also known as CMG2 (capillary morphogenesis gene 2 protein) is a transmembrane protein that is induced during capillary morphogenesis. It contains a vWA domain that shows strong binding to laminin and collagen IV, suggesting that this protein plays a role in basement membrane matrix assembly and endothelial cell morphogenesis (PMID: 11683410; 14508707). Like its paralog TEM8 (ANTXR1), ANTXR2 also functions as a receptor for anthrax toxin. Mutations in ANTXR2 result in the allelic disorders juvenile hyaline fibromatosis and infantile systemic hyalinosis (PMID: 14508707; 12973667; 22383261).

Publications

Species	Application	Title
human	WB	Angiogenesis; Capillary morphogenesis gene 2 (CMG2) mediates growth factor-induced angiogenesis by regulating endothelial cell chemotaxis.; Authors - Lorna M Cryan ; KO Validated
human	WB,IHC,CoIP	Oncogene; Capillary morphogenesis gene 2 maintains gastric cancer stem-like cell phenotype by activating a Wnt/β-catenin pathway.; Authors - Chengdong Ji
human	IHC	J Pathol; Capillary morphogenesis protein 2 is a novel prognostic biomarker and plays oncogenic roles in glioma.; Authors - Juan Tan
human	IHC	FASEB J; Genetic deletion of CMG2 exacerbates systemic-to-pulmonary shunt-induced pulmonary arterial hypertension.; Authors - Liukun Meng
Camelus bactrianus	WB	Reprod Domest Anim; Screening for reproductive biomarkers in Bactrian camel via iTRAQ analysis of proteomes.; Authors - Qi Wang
human	WB,IP	Proc Natl Acad Sci U S A; Anthrax toxin requires ZDHHC5-mediated palmitoylation of its surface-processing host enzymes.; Authors - Oksana A Sergeeva

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