Anticorps Polyclonal de lapin anti-Annexin A1

• Phare
• Validé par KD/KO

Annexin A1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

29

Avis (1)

N° de cat : 21990-1-AP

Synonymes

Annexin 1, annexin A1, Annexin I, ANX1, ANXA1, Calpactin 2, Calpactin II, Chromobindin 9, Lipocortin I, LPC1

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Galerie de données de validation

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  WB analysis using 21990-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21990-1-AP (Annexin A1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21990-1-AP

  WB result of Annexin A1 antibody (21990-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Annexin A1 transfected HeLa cells.

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  WB analysis of multi-cells using 21990-1-AP

  WB result of 21990-1-AP (Annexin A1 antibody).

• 

  WB analysis of C6 using 21990-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 21990-1-AP (Annexin A1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 21990-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 21990-1-AP (Annexin A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 21990-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 21990-1-AP (Annexin A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 21990-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21990-1-AP (Annexin A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 21990-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21990-1-AP (Annexin A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-453s using 21990-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 21990-1-AP (Annexin A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 21990-1-AP

  IP Result of anti-Annexin A1 (IP:21990-1-AP, 3ug; Detection:21990-1-AP 1:2000) with HeLa cells lysate 2400ug.

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  IHC staining of human oesophagus using 21990-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 21990-1-AP (Annexin A1 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human oesophagus using 21990-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 21990-1-AP (Annexin A1 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human small intestine using 21990-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 21990-1-AP (Annexin A1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human small intestine using 21990-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 21990-1-AP (Annexin A1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung using 21990-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 21990-1-AP (Annexin A1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 21990-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 21990-1-AP (Annexin A1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary tumor using 21990-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 21990-1-AP (Annexin A1 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human ovary tumor using 21990-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 21990-1-AP (Annexin A1 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human oesophagus using 21990-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 21990-1-AP (Annexin A1 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human oesophagus using 21990-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 21990-1-AP (Annexin A1 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human kidney using 21990-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21990-1-AP (Annexin A1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 21990-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21990-1-AP (Annexin A1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of A431 using 21990-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Annexin A1 antibody (21990-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A431 using 21990-1-AP

  Immunofluorescent analysis of A431 cells using 21990-1-AP (Annexin A1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A549 using 21990-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Annexin A1 antibody (21990-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of NIH/3T3 using 21990-1-AP

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.2 ug Anti-Human Annexin A1 (21990-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules A549, cellules C6, cellules HeLa, cellules HepG2, cellules K-562, cellules MCF-7, cellules MDA-MB-453s, cellules NIH/3T3, cellules U-937, multi-cellules
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu œsophagien humain, tissu de tumeur ovarienne humain, tissu d'intestin grêle humain, tissu pulmonaire humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A549, cellules A431
• Résultats positifs en cytométrie: cellules NIH/3T3,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 23 publications below
• IHC: See 5 publications below
• IF: See 8 publications below
• IP: See 4 publications below

Informations sur le produit

21990-1-AP cible Annexin A1 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Annexin A1 Protéine recombinante Ag17273
• Nom complet: annexin A1
• Masse moléculaire calculée: 346 aa, 39 kDa
• Poids moléculaire observé: 37 kDa
• Numéro d’acquisition GenBank: BC001275
• Symbole du gène: ANXA1
• Identification du gène (NCBI): 301
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ANXA1, also named as ANX1, LPC1 and p35, belongs to the annexin family. It is a member of the annexin family of Ca2+-binding and phospholipidbinding proteins, and it is particularly abundant in various populations of peripheral blood leukocytes. ANXA1 regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. ANXA1 is a pleiotrophic protein produced by many cell types including peripheral blood leucocytes.

Publications

Species	Application	Title
mouse	WB,IF	Nature; Evolutionarily divergent mTOR remodels translatome for tissue regeneration; Authors - Olena Zhulyn
	IP	Cell Host Microbe; Global mapping of Salmonella enterica-host protein-protein interactions during infection.; Authors - Philipp Walch
human	WB	Oncogene; The E3 ligase RBCK1 reduces the sensitivity of ccRCC to sunitinib through the ANKRD35-MITD1-ANXA1 axis; Authors - Yapeng Wang ; KD Validated
human	WB,IHC,IF,IP	Elife; ER-luminal [Ca2+] regulation of InsP3 receptor gating mediated by an ER-luminal peripheral Ca2+-binding protein.; Authors - Horia Vais
human	WB	Mol Ther; Membrane Stabilization by Modified Steroid Offers a Potential Therapy for Muscular Dystrophy Due to Dysferlin Deficit.; Authors - Sen Chandra Sreetama
human	WB	Cancer Immunol Immunother; Circulating PD-L1 is associated with T cell infiltration and predicts prognosis in patients with CRLM following hepatic resection.; Authors - Xiuxing Chen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Guorong (Verified Customer) (05-23-2022)

excellent with 293T cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: 293T

Annexin A1 Antibody Western Blot validation (1:2000 dilution) in 293T (Cat no:21990-1-AP)

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C