Anticorps Monoclonal anti-Annexin A1

• Phare
• Validé par KD/KO

Annexin A1 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

5

CloneNo.

1E1B7

N° de cat : 66344-1-Ig

Synonymes

Annexin 1, annexin A1, Annexin I, ANX1, ANXA1, Calpactin 2, Calpactin II, Chromobindin 9, Lipocortin I, LPC1, p35

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Galerie de données de validation

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  WB analysis using 66344-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66344-1-Ig (Annexin A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66344-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66344-1-Ig (Annexin A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66344-1-Ig

  WB result of Annexin A1 antibody (66344-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Annexin A1 transfected HeLa cells.

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  WB analysis of NIH/3T3 using 66344-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66344-1-Ig (Annexin A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of 4T1 using 66344-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 66344-1-Ig (Annexin A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66344-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66344-1-Ig (Annexin A1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 66344-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66344-1-Ig (Annexin A1 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human stomach using 66344-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66344-1-Ig (Annexin A1 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse liver using 66344-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66344-1-Ig (Annexin A1 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 66344-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66344-1-Ig (Annexin A1 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 66344-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66344-1-Ig (Annexin A1 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas using 66344-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66344-1-Ig (Annexin A1 antibody at dilution of 1:200 (under 40x lens).

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  IF Staining of A549 using 66344-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed A549 cells using 66344-1-Ig(Annexin A1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of K-562 using 66344-1-Ig

  1X10^6 K-562 cells were intracellularly stained with 0.8 ug Anti-Human Annexin A1 (66344-1-Ig, Clone:1E1B7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.8 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules 4T1, cellules A431, cellules HCT 116, cellules HeLa, cellules HepG2, cellules Jurkat, cellules K-562, cellules NIH/3T3
• Résultats positifs en IHC: tissu d'estomac humain, tissu hépatique de souris, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A549
• Résultats positifs en cytométrie: cellules K-562

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below
• IF: See 3 publications below

Informations sur le produit

66344-1-Ig cible Annexin A1 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Annexin A1 Protéine recombinante Ag17273
• Nom complet: annexin A1
• Masse moléculaire calculée: 346 aa, 39 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC001275
• Symbole du gène: ANXA1
• Identification du gène (NCBI): 301
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ANXA1, also named as ANX1, LPC1 and p35, belongs to the annexin family. It is a member of the annexin family of Ca2+-binding and phospholipidbinding proteins, and it is particularly abundant in various populations of peripheral blood leukocytes. ANXA1 regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. ANXA1 is a pleiotrophic protein produced by many cell types including peripheral blood leucocytes.

Publications

Species	Application	Title
human	IF	Matrix Biol; Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.; Authors - Emna Ouni
human	IHC	J Mater Chem B; Magnetic hyperthermia induces effective and genuine immunogenic tumor cell death with respect to exogenous heating; Authors - Bin Yan
human	IF	Nat Commun; Annexin A1 is a polarity cue that directs mitotic spindle orientation during mammalian epithelial morphogenesis; Authors - Maria Fankhaenel
mouse		J Proteome Res; Effect of Tamoxifen on Proteome Expression during In Vitro Myogenesis in Murine Skeletal Muscle C2C12 Cells; Authors - Emily A Morris
human	IF	Nature; Stress granules plug and stabilize damaged endolysosomal membranes; Authors - Claudio Bussi

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C