Anticorps Polyclonal de lapin anti-Annexin VI

• Phare
• Validé par KD/KO

Annexin VI Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(13)

Avis (1)

N° de cat : 12542-1-AP

Synonymes

Annexin A6, ANXA6, 67 kDa calelectrin, Annexin 6, Annexin-6

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Galerie de données de validation

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  WB analysis using 12542-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12542-1-AP (Annexin VI antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12542-1-AP

  WB result of Annexin VI antibody (12542-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Annexin VI transfected HeLa cells.

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  WB analysis of human brain using 12542-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12542-1-AP (Annexin VI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 12542-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12542-1-AP (Annexin VI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 12542-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12542-1-AP (Annexin VI antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12542-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12542-1-AP (Annexin VI antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12542-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12542-1-AP (Annexin VI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12542-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12542-1-AP (Annexin VI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse placenta using 12542-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 12542-1-AP (Annexin VI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 12542-1-AP

  IP result of anti-Annexin VI (IP:12542-1-AP, 4ug; Detection:12542-1-AP 1:2000) with HeLa cells lysate 1200 ug.

• 

  IP experiment of mouse kidney using 12542-1-AP

  IP result of anti-Annexin VI (IP:12542-1-AP, 4ug; Detection:12542-1-AP 1:2000) with mouse kidney tissue lysate 3400 ug.

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  IHC staining of human colon using 12542-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12542-1-AP (Annexin VI antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 12542-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12542-1-AP (Annexin VI antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon using 12542-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12542-1-AP (Annexin VI antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 12542-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12542-1-AP (Annexin VI antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 12542-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12542-1-AP (Annexin VI antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human kidney using 12542-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12542-1-AP (Annexin VI antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 12542-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12542-1-AP (Annexin VI antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 12542-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 12542-1-AP (Annexin VI antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules Jurkat, tissu cérébral de souris, tissu cérébral humain, tissu placentaire de souris, tissu rénal de souris
• Résultats positifs en IP: cellules HeLa, tissu rénal de souris
• Résultats positifs en IHC: tissu de côlon humain, tissu rénal humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 13 publications below
• IHC: See 3 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Informations sur le produit

12542-1-AP cible Annexin VI dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Annexin VI Protéine recombinante Ag3263
• Nom complet: annexin A6
• Masse moléculaire calculée: 673 aa, 76 kDa
• Poids moléculaire observé: 67 kDa
• Numéro d’acquisition GenBank: BC017046
• Symbole du gène: Annexin VI
• Identification du gène (NCBI): 309
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ANXA6, also called CBP68, or 67 kDa calelectrin, belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis.

Publications

Species	Application	Title
mouse	WB	Adv Sci (Weinh); Isolation and Comprehensive Analysis of Cochlear Tissue-Derived Small Extracellular Vesicles; Authors - Pei Jiang
mouse	WB	J Extracell Vesicles; Distinguishing functional exosomes and other extracellular vesicles as a nucleic acid cargo by the anion-exchange method.; Authors - Naohiro Seo
human	WB	Oncotarget; CD300A promotes tumor progression by PECAM1, ADCY7 and AKT pathway in acute myeloid leukemia.; Authors - Xiaogang Sun
rat	WB	Front Cell Dev Biol; Dynamic Mitochondrial Proteome Under Polyamines Treatment in Cardiac Aging.; Authors - Hao Zhang
rat	WB,IF	Proteome Sci; TMT-based proteomics analysis of the cerebral cortex of TauT knockout rats.; Authors - Yiming Xia
rat	WB	Proteome Sci; A dynamic plasma membrane proteome analysis of alcohol-induced liver cirrhosis.; Authors - Jia Xiaofang X

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Gaurav (Verified Customer) (02-29-2024)

Very good antibody with expected band at appropriate position. Works well in TBST, 5% Skimmed milk and 3% BSA in TBST

• Applications: Western Blot, Immunoprecipitation, Cell culture
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Smooth Muscle cells, Aorta, Placental Trophoblasts.

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