Anticorps Polyclonal de lapin anti-AP1B1

AP1B1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 16932-1-AP

Synonymes

ADTB1, AP 1 complex subunit beta 1, AP105A, AP1B1, BAM22, Beta 1 adaptin, Beta adaptin 1, CLAPB2

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Galerie de données de validation

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  WB analysis of HEK-293 using 16932-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16932-1-AP (AP1B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 16932-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16932-1-AP (AP1B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 16932-1-AP

  IP result of anti-AP1B1 (IP:16932-1-AP, 4ug; Detection:16932-1-AP 1:300) with HEK-293 cells lysate 2320 ug.

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  IF Staining of HepG2 using 16932-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 16932-1-AP (AP1B1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below

Informations sur le produit

16932-1-AP cible AP1B1 dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AP1B1 Protéine recombinante Ag10417
• Nom complet: adaptor-related protein complex 1, beta 1 subunit
• Masse moléculaire calculée: 919 aa, 101 kDa
• Poids moléculaire observé: 101 kDa
• Numéro d’acquisition GenBank: BC046242
• Symbole du gène: AP1B1
• Identification du gène (NCBI): 162
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AP1B1 is a subunit of the adaptor complex AP-1 and is a member of the adaptin protein family. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP-1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors.

Publications

Species	Application	Title
human	WB	Nat Genet; Bidirectional genome-wide CRISPR screens reveal host factors regulating SARS-CoV-2, MERS-CoV and seasonal HCoVs; Authors - Antoine Rebendenne
human	WB	Am J Hum Genet; Recessive Mutations in AP1B1 Cause Ichthyosis, Deafness, and Photophobia.; Authors - Lynn M Boyden
human	WB	J Cell Biol; Cargo-selective SNX-BAR proteins mediate retromer trimer independent retrograde transport.; Authors - Arunas Kvainickas
Human		Sci Rep; In vivo monitoring of the recruitment and activation of AP-1 by Arf1.; Authors - Etienne Sauvageau
human	WB	Mol Genet Metab; Clinical, biochemical and cell biological characterization of KIDAR syndrome associated with a novel AP1B1 variant; Authors - Tarun Kaniganti
mouse	WB	Nat Commun; Synaptic vesicle-omics in mice captures signatures of aging and synucleinopathy; Authors - Virginia Gao

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