Anticorps Polyclonal de lapin anti-AP1M1

• Phare
• Validé par KD/KO

AP1M1 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(10)

N° de cat : 12112-1-AP

Synonymes

AP 1 complex subunit mu 1, AP mu chain family member mu1A, AP1M1, AP47, CLAPM2, CLTNM, MU 1A, Mu adaptin 1, Mu1A adaptin

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Galerie de données de validation

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  WB analysis of Hacat cells using 12112-1-AP

  WB resut of AP1M1 antibody (12112-1-AP, 1:500) with siRNA cell lysate.

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  WB analysis of human brain using 12112-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12112-1-AP (AP1M1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 12112-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 12112-1-AP (AP1M1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 12112-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 12112-1-AP (AP1M1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 12112-1-AP

  IP result of anti-AP1M1 (IP:12112-1-AP, 4ug; Detection:12112-1-AP 1:500) with SH-SY5Y cells lysate 2500ug.

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, cellules HaCaT
• Résultats positifs en IP: cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 9 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

12112-1-AP cible AP1M1 dans les applications de WB, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: canin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AP1M1 Protéine recombinante Ag2757
• Nom complet: adaptor-related protein complex 1, mu 1 subunit
• Masse moléculaire calculée: 423 aa, 49 kDa
• Poids moléculaire observé: 49 kDa
• Numéro d’acquisition GenBank: BC017469
• Symbole du gène: AP1M1
• Identification du gène (NCBI): 8907
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AP1M1, also named as CLTNM, Mu-adaptin 1 and Clathrin coat assembly protein AP47, belongs to the adaptor complexes medium subunit family. It is a subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; Plasma membrane contributes to the formation of pre-autophagosomal structures.; Authors - Ravikumar Brinda B ; KD Validated
human	IF,CoIP	Int J Mol Sci; Leucine Zipper Downregulated in Cancer-1 Interacts with Clathrin Adaptors to Control Epidermal Growth Factor Receptor (EGFR) Internalization and Gefitinib Response in EGFR-Mutated Non-Small Cell Lung Cancer; Authors - Hsien-Neng Huang
human	WB,IF	Traffic; AP-1A controls secretory granule biogenesis and trafficking of membrane secretory granule proteins.; Authors - Mathilde Bonnemaison ; KD Validated
	WB	J Biol Chem; Adaptor Protein-1 Complex Affects the Endocytic Trafficking and Function of Peptidylglycine α-Amidating Monooxygenase, a Luminal Cuproenzyme.; Authors - Mathilde L Bonnemaison ; KD Validated
human,mouse	WB	Biomed Pharmacother; Dioscin alleviates Alzheimer's disease through regulating RAGE/NOX4 mediated oxidative stress and inflammation.; Authors - Linshu Guan
	WB	J Biol Chem; Clathrin adaptor AP-1-mediated Golgi export of amyloid precursor protein is crucial for the production of neurotoxic amyloid fragments.; Authors - Yunan C Januário

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