Anticorps Polyclonal de lapin anti-AP2B1

• Phare
• Validé par KD/KO

AP2B1 Polyclonal Antibody for IF, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IF, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 15690-1-AP

Synonymes

ADTB2, AP 2 complex subunit beta, AP105B, AP2 BETA, AP2B1, Beta 2 adaptin, Beta adaptin, CLAPB1

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Galerie de données de validation

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  WB analysis using 15690-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15690-1-AP (AP2B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 15690-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15690-1-AP (AP2B1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 15690-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 15690-1-AP (AP2B1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 15690-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15690-1-AP (AP2B1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 15690-1-AP

  IP Result of anti-AP2B1 (IP:15690-1-AP, 4ug; Detection:15690-1-AP 1:500) with K-562 cells lysate 3200ug.

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  IF Staining of HeLa using 15690-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15690-1-AP (AP2B1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules C6, cellules K-562, cellules NIH/3T3, cellules SH-SY5Y
• Résultats positifs en IP: cellules K-562
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IF: See 2 publications below

Informations sur le produit

15690-1-AP cible AP2B1 dans les applications de WB, IP, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AP2B1 Protéine recombinante Ag8248
• Nom complet: adaptor-related protein complex 2, beta 1 subunit
• Masse moléculaire calculée: 105 kDa
• Poids moléculaire observé: 100-105 kDa
• Numéro d’acquisition GenBank: BC006201
• Symbole du gène: AP2B1
• Identification du gène (NCBI): 163
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AP2B1 is a component of the adaptor protein complex 2 (AP-2). AP complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP complexes are involved in the formation of clathrin-coated vesicles (CCVs) by recruiting the scaffold protein, clathrin. AP complexes also play a pivotal role in the cargo selection by recognizing the sorting signals within the cytoplasmic tail of integral membrane proteins. AP-2 works on the plasma membrane to internalize cargo in clathrin-mediated endocytosis.

Publications

Species	Application	Title
pig	WB	Emerg Microbes Infect; CD1d facilitates African swine fever virus entry into the host cells via clathrin-mediated endocytosis; Authors - Xin Chen
	WB	Elife; Syncytin-mediated open-ended membrane tubular connections facilitate the intercellular transfer of cargos including Cas9 protein; Authors - Congyan Zhang
pig	WB,IF	Sci China Life Sci; NPC1-regulated dynamic of clathrin-coated pits is essential for viral entry.; Authors - Guoli Li
human	WB	J Virol; The G protein-coupled receptor FFAR2 promotes internalization during influenza A virus entry.; Authors - Guangwen Wang ; KD Validated
pig	WB	Viruses; Pseudorabies Virus Inhibits Expression of Liver X Receptors to Assist Viral Infection.; Authors - Yi Wang
human	WB	J Neuropathol Exp Neurol; Alzheimer Disease Pathology-Associated Polymorphism in a Complex Variable Number of Tandem Repeat Region Within the MUC6 Gene, Near the AP2A2 Gene.; Authors - Yuriko Katsumata

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