Anticorps Polyclonal de lapin anti-APEX1

• Phare
• Validé par KD/KO

APEX1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(29)

N° de cat : 10203-1-AP

Synonymes

APE, APE1, APE-1, APEN, APEX

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Galerie de données de validation

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  WB analysis of HeLa using 10203-1-AP

  WB result of APEX1 antibody (10203-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-APEX1 transfected HeLa cells.

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  WB analysis of HeLa using 10203-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10203-1-AP (APEX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HuH-7 using 10203-1-AP

  WB result of APEX1 antibody (10203-1-AP; 1:10000; room temperature for 1.5 hours) with wild-type and APEX1 knockout HuH-7 cells.

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  WB analysis of mouse brain using 10203-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10203-1-AP (APEX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10203-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10203-1-AP (APEX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10203-1-AP

  IP result of anti-APEX1 (IP:10203-1-AP, 4ug; Detection:10203-1-AP 1:1000) with HeLa cells lysate 1600ug.

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  IHC staining of human stomach cancer using 10203-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10203-1-AP (APEX1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 10203-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10203-1-AP (APEX1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 10203-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10203-1-AP (APEX1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 10203-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 10203-1-AP (APEX1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 10203-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using APEX1 antibody (10203-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HuH-7, tissu cérébral de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, tissu de cancer de la thyroïde humain, tissu de côlon de rat, tissu de côlon de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 8 publications below
• WB: See 22 publications below
• IHC: See 5 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

10203-1-AP cible APEX1 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: APEX1 Protéine recombinante Ag0251
• Nom complet: APEX nuclease (multifunctional DNA repair enzyme) 1
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC002338
• Symbole du gène: APEX1
• Identification du gène (NCBI): 328
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

APEX1, also named as APE, APE1, HAP1 and REF-1, belongs to the DNA repair enzymes AP/ExoA family. It is a multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 are in DNA repair and redox regulation of transcriptional factors. APEX nuclease is a DNA repair enzyme having apurinic/apyrimidinic (AP) endonuclease, 3-prime,5-prime-exonuclease, DNA 3-prime repair diesterase, and DNA 3-prime-phosphatase activities. On the other hand, APEX1 also exerts reversible nuclear redox activity to regulate DNA binding affinity and transcriptional activity of transcriptional factors by controlling the redox status of their DNA-binding domain, such as the FOS/JUN AP-1 complex after exposure to IR. APEX1 is involved in calcium-dependent down-regulation of parathyroid hormone (PTH) expression by binding to negative calcium response elements (nCaREs). When acetylated at Lys-6 and Lys-7, APEX1 stimulates the YBX1-mediated MDR1 promoter activity, leading to drug resistance. It also acts as an endoribonuclease involved in the control of single-stranded RNA metabolism. It plays a role in regulating MYC mRNA turnover by preferentially cleaving in between UA and CA dinucleotides of the MYC coding region determinant (CRD). In association with NMD1, APEX1 plays a role in the rRNA quality control process during cell cycle progression. 10203-1-AP is a rabbit polyclonal antibody raised against full length APE1 of human origin.

Publications

Species	Application	Title
human	WB	Sci Adv; Deficiency in mammalian STN1 promotes colon cancer development via inhibiting DNA repair; Authors - Dinh Duc Nguyen
mouse	WB	Cell Death Dis; DNA polymerase beta connects tumorigenicity with the circadian clock in liver cancer through the epigenetic demethylation of Per1; Authors - Siyu Chen
human	WB,IHC	Mol Ther; miR-1293, a Candidate for miRNA-Based Cancer Therapeutics, Simultaneously Targets BRD4 and the DNA Repair Pathway.; Authors - Yuki Takagawa ; KD Validated
rat	IF	Free Radic Biol Med; Systematical exploration of transcription factors in cardiomyocytes against hydrogen peroxide-induced oxidative stress by Danhong injection.; Authors - Jingjing Zhang
human	WB,IP	Int Immunopharmacol; Inhibitors of APE1 redox and ATM synergistically sensitize osteosarcoma cells to ionizing radiation by inducing ferroptosis; Authors - Hanxi Xiao ; KD Validated
human	WB	J Ethnopharmacol; Integrated network pharmacology, proteomics, molecular docking, and experiments in vivo and in vitro to explore the efficacy and potential mechanism of bufalin against hepatocellular carcinoma angiogenesis; Authors - Yuanchao Li

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