Anticorps Polyclonal de lapin anti-Apolipoprotein AI

Apolipoprotein AI Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

32

Avis (1)

N° de cat : 14427-1-AP

Synonymes

Apo AI, ApoA I, APOA1, apolipoprotein A I, Apolipoprotein A1

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Galerie de données de validation

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  WB analysis of human plasma using 14427-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 14427-1-AP (Apolipoprotein AI antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human ileum using 14427-1-AP

  human ileum tissue were subjected to SDS PAGE followed by western blot with 14427-1-AP (Apolipoprotein AI antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 14427-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14427-1-AP (Apolipoprotein AI antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 14427-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14427-1-AP (Apolipoprotein AI antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14427-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14427-1-AP (Apolipoprotein AI antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 14427-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14427-1-AP (Apolipoprotein AI antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 14427-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14427-1-AP (Apolipoprotein AI antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human lung using 14427-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14427-1-AP (Apolipoprotein AI antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 14427-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14427-1-AP (Apolipoprotein AI antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 14427-1-PBS

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Apolipoprotein AI antibody (14427-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 14427-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Apolipoprotein AI antibody (14427-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 14427-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug Apolipoprotein AI antibody (14427-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: tissu sanguin humain, plasma humain, tissu cérébral humain, tissu d'iléon humain
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu hépatique humain, tissu pulmonaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2, cellules HeLa
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 23 publications below
• IHC: See 10 publications below
• IF: See 4 publications below
• IP: See 2 publications below

Informations sur le produit

14427-1-AP cible Apolipoprotein AI dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Apolipoprotein AI Protéine recombinante Ag5793
• Nom complet: apolipoprotein A-I
• Masse moléculaire calculée: 31 kDa
• Poids moléculaire observé: 26-30 kDa
• Numéro d’acquisition GenBank: BC005380
• Symbole du gène: APOA1
• Identification du gène (NCBI): 335
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ApoA1 is a major protein component of high density lipoproteins (HDL) which is associated with reversed cholesterol transport, lipid/cholesterol binding, lecithin/cholesterol acyltransferase (LCAT) activation and specific receptors binding. It is synthesized in the liver and small intestine. Defects of ApoA1 cause low HDL level and systemic non-neuropathic amyloidosis. Serum concentration of ApoA1 is inversely related to the risk of developing atherosclerosis. This antibody was generated against the C-terminal region of human ApoA1.

Publications

Species	Application	Title
	IP	Science; Enterically derived high-density lipoprotein restrains liver injury through the portal vein.; Authors - Yong-Hyun Han
mouse	WB,IP	Neuron; Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.; Authors - Xiaohui Li
human	IHC	Br J Cancer; A new panel of pancreatic cancer biomarkers discovered using a mass spectrometry-based pipeline.; Authors - Xiaohui Liu
human	WB	Theranostics; Exosome-mediated delivery of inflammation-responsive Il-10 mRNA for controlled atherosclerosis treatment; Authors - Te Bu
		Br J Pharmacol; A novel apoA-I mimetic peptide suppresses atherosclerosis by promoting physiological HDL function in apoE-/- mice.; Authors - Sanhu Gou
human	IHC	Am J Obstet Gynecol; Trophoblast apoptosis from pregnancies complicated by fetal growth restriction is associated with enhanced p53 expression.; Authors - Roni Levy

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Alexandre (Verified Customer) (02-28-2019)

Detection of both human and mouse APOA1 protein by westernblotImmunoprecipitation of both human and mouse APOA1

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: WB 1/1000
• Cell Tissue Type: HepG2 / Mouse Liver

Apolipoprotein AI Antibody Western Blot,Immunoprecipitation, validation (WB 1/1000 dilution) in HepG2 / Mouse Liver (Cat no:14427-1-AP)