Anticorps Polyclonal de lapin anti-Apolipoprotein A II/APOA2

Apolipoprotein A II/APOA2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 16845-1-AP

Synonymes

APOA2, apolipoprotein A II, ApoA-II, Apo-AII, ApoA II

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Galerie de données de validation

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  WB analysis of human plasma using 16845-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 16845-1-AP (Apolipoprotein A II/APOA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human plasma using 16845-1-AP

  human serum were subjected to SDS PAGE followed by western blot with 16845-1-AP (Apolipoprotein A II antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of human plasma using 16845-1-AP

  IP result of anti-Apolipoprotein A II/APOA2 (IP:16845-1-AP, 4ug; Detection:16845-1-AP 1:500) with human plasma lysate 4000 ug.

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  IHC staining of human liver using 16845-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16845-1-AP (Apolipoprotein A II/APOA2 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 16845-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16845-1-AP (Apolipoprotein A II antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon using 16845-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16845-1-AP (Apolipoprotein A II/APOA2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 16845-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16845-1-AP (Apolipoprotein A II/APOA2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 16845-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 16845-1-AP (Apolipoprotein A II antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 16845-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 16845-1-AP (Apolipoprotein A II antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 16845-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Apolipoprotein A II/APOA2 antibody (16845-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: tissu plasmatique humain,
• Résultats positifs en IP: tissu plasmatique humain,
• Résultats positifs en IHC: tissu hépatique humain, tissu de cirrhose hépatique humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

16845-1-AP cible Apolipoprotein A II/APOA2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Apolipoprotein A II/APOA2 Protéine recombinante Ag9863
• Nom complet: apolipoprotein A-II
• Masse moléculaire calculée: 100 aa, 11 kDa
• Poids moléculaire observé: 7-9 kDa (monomer), 15 kDa (dimer)
• Numéro d’acquisition GenBank: BC005282
• Symbole du gène: APOA2
• Identification du gène (NCBI): 336
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Apolipoprotein A-II (APOA2) is a major component of high density lipoprotein (HDL) and it plays an important role in directing the fate of lipid metabolism among HDL. It is primarily synthesized by liver. The predicted MW of ApoA2 is 11 kDa, while the mature form is smaller (7-10 kDa) since the signal peptide was cleaved. In humans, most of circulating apoA2 exist as dimer. Five types of APOA2 dimer exist: homodimer apoA2-ATQ/ATQ, heterodimer apoA2-ATQ/AT, homodimer apoA2-AT/AT, apoA2-AT/A, and apoA2-A/A. ApoA2 isoforms are considered to be some of the most promising serum/plasma biomarkers for aiding the early detection of pancreatic cancer. (PMID: 29081441, 29481802)

Publications

Species	Application	Title
human	WB	Nat Commun; N1-methyladenosine methylation in tRNA drives liver tumourigenesis by regulating cholesterol metabolism.; Authors - Yanying Wang
human	WB	Exp Cell Res; Extracellular vesicle-mediated transfer of the lncRNA-TC0101441 promotes endometriosis migration/invasion.; Authors - Jun-Jun Qiu
human,mouse	WB	Int J Mol Sci; Recombinant Humanized IgG1 Antibody Promotes Reverse Cholesterol Transport through FcRn-ERK1/2-PPARα Pathway in Hepatocytes; Authors - Zhonghao Li

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