Anticorps Polyclonal de lapin anti-APOBEC3B

APOBEC3B Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 14559-1-AP

Synonymes

A3B, DNA dC->dU-editing enzyme APOBEC-3B, EC:3.5.4.38, Phorbolin 1 related protein, Phorbolin 2/3

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Galerie de données de validation

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  WB analysis using 14559-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14559-1-AP (APOBEC3B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected HEK-293 using 14559-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 14559-1-AP (APOBEC3B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 5 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

14559-1-AP cible APOBEC3B dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: APOBEC3B Protéine recombinante Ag6115
• Nom complet: apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3B
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC053859
• Symbole du gène: APOBEC3B
• Identification du gène (NCBI): 9582
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The DNA cytosine deaminase APOBEC3B was identified recently as a source of DNA damage and mutagenesis in breast, head/neck, cervix, bladder, lung, ovary, and to lesser extents additional cancer types. This enzyme is also an effector protein in the innate immune response to virus infection. APOBEC3B has 3 isoforms produced by alternative splicing with the MW of 46, 29, 43 kDa. It can also exist as a dimer and can interact with APOBEC3G.

Publications

Species	Application	Title
human	IHC	J Med Genet; Multiregion whole-genome sequencing depicts intratumour heterogeneity and punctuated evolution in ovarian clear cell carcinoma.; Authors - Xia Yin
human	IHC	Front Oncol; Correlation Between APOBEC3B Expression and Clinical Characterization in Lower-Grade Gliomas.; Authors - Hao Zhang
human	WB,IP,IHC	Mol Carcinog; HPV-16/18 E6-induced APOBEC3B expression associates with proliferation of cervical cancer cells and hypomethylation of Cyclin D1.; Authors - Qiong Fan
mouse,human	WB,IHC	Zhongguo Fei Ai Za Zhi; [SWI/SNF Complex Gene Mutations Promote the Liver Metastasis of Non-small Cell Lung Cancer Cells in NSI Mice]; Authors - Lingling Gao

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